Comparative Study on Bone Marrow-Versus Adipose-Derived Stem Cells on Regeneration and Re-Innervation of Skeletal Muscle
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Online ISSN 2212-5469
ORIGINAL ARTICLE
Comparative Study on Bone Marrow-Versus Adipose-Derived Stem Cells on Regeneration and Re-Innervation of Skeletal Muscle Injury in Wistar Rats Manal H. Moussa1 • Ghada G. Hamam1 • Asmaa E. Abd Elaziz1 • Marwa A. Rahoma1 • Abeer A. Abd El Samad1 • Dalia A. A. El-Waseef1 Mohamed A. Hegazy1
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Received: 12 June 2020 / Revised: 18 July 2020 / Accepted: 21 July 2020 Ó The Korean Tissue Engineering and Regenerative Medicine Society 2020
Abstract BACKGROUND: Skeletal muscle injuries are frequent clinical challenges due to associated fibrosis and disability. Regenerative medicine is an emerging promising strategy for such cases. The aim of this study was to compare between the effects of bone marrow-mesenchymal stem cells (BM-MSCs) versus adipose tissue stromal cells (ADSCs) on regeneration and re-innervation of skeletal muscle laceration injury in Wistar rats at different time intervals. METHODS: Six young male rats were used as a source of allogenic MSCs. Eighty-four adult female rats were divided into: Group I (control), Group II (Untreated Laceration): right gluteal muscle was lacerated and left for spontaneous healing, Group III (BM-MSCs): right gluteal muscle was lacerated with concomitant local intramuscular injection of 1 9 106 BM-MSCs in the lacerated muscle, Group IV (ADSCs): right gluteal muscle was lacerated with concomitant local intramuscular injection of 1 9 106 ADSCs in lacerated muscle. Rats were sacrificed after one, two and eight weeks. Muscles were processed to prepare sections stained with H&E, Mallory’s trichrome and immune-histochemical staining (neurofilament light chain). RESULTS: A significant increase in collagen fibers and failure of re-innervation were noticed in untreated laceration group. BM-MSCs-treated groups showed regeneration of muscle fibers but with increased collagen fibers. Meanwhile, ADSCs showed better regenerative effects evidenced by significant increase in the number of myotubes and significant decrease in collagen deposition. Re-innervation was noticed in MSCs-injected muscles after 8 weeks of laceration. CONCLUSION: Both BM-MSCs and ADSCs improved regeneration of skeletal muscle laceration injury at short- and long-term durations. However, fibrosis was less in ADSCs-treated rats. Effective re-innervation of injured muscles occurred only at the long-term duration. Keywords Muscle laceration Bone marrow-derived mesenchymal stem cells Adipose tissue-derived stem cells Reinnervation Neurofilament light chain
1 Introduction
& Ghada G. Hamam [email protected]; [email protected] 1
Department of Histology and Cell Biology, Faculty of Medicine, Ain Shams University, El-Khalyfa El-Mamoun Street Abbasya, Cairo, Egypt
Skeletal muscle is a dynamic tissue that is responsible for voluntary movement and postural maintenance. It is the most abundant tissue in the human body, representing approximately 40% of body mass [1]. Skeletal muscle injury can be encountered clinically as contusion, strain, laceration or a combination of
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