Biosensors and Biodetection Methods and Protocols Volume 1: Optical-
This volume provides comprehensive and detailed technical protocols on current biosensor and biodetection technologies and examples of their applications and capabilities. Chapters in Biosensors and Biodetection: Methods and Protocols, Volume 1:
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Avraham Rasooly Ben Prickril Editors
Biosensors and Biodetection Methods and Protocols Volume 1: Optical-Based Detectors Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Biosensors and Biodetection Methods and Protocols Volume 1: Optical-Based Detectors Second Edition Edited by
Avraham Rasooly National Cancer Institute National Institutes of Health Rockville, MD, USA
Ben Prickril National Cancer Institute National Institutes of Health Rockville, MD, USA
Editors Avraham Rasooly National Cancer Institute National Institutes of Health Rockville, MD, USA
Ben Prickril National Cancer Institute National Institutes of Health Rockville, MD, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6846-6 ISBN 978-1-4939-6848-0 (eBook) DOI 10.1007/978-1-4939-6848-0 Library of Congress Control Number: 2017932742 © Springer Science+Business Media LLC 2009, 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
This book is dedicated to the memory of Jury Rasooly Ph.D., Malkah Rasooly, and Ilan Rasooly.
Preface Biosensor Technologies A biosensor is defined by the International Union of Pure and Applied Chemistry (IUPAC) as “a device that uses specific biochemical reactions mediated by isolated enzymes, immunosystems, tissues, organelles or whole cells to detect chemical compounds usually by electrical, thermal or optical signals” [1]; all biosensors are based on a two-component system: 1. Biological recognition element
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