Alpha-1 Antitrypsin Deficiency Methods and Protocols
This volume provides protocols that expand on the latest alpha-1 antitrypsin (AAT) research. The chapters in this book are divided in to three sections: Part I is dedicated to patient-oriented research; part II discusses animal models; and Part III focuse
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Florie Borel · Christian Mueller Editors
Alpha-1 Antitrypsin Deficiency Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Alpha-1 Antitrypsin Deficiency Methods and Protocols
Edited by
Florie Borel Horae Gene Therapy Center, University of Massachusetts Medical School, Worcester, MA, USA
Christian Mueller Horae Gene Therapy Center, University of Massachusetts Medical School, Worcester, MA, USA; Department of Pediatrics, University of Massachusetts Medical School, Worcester, MA, USA
Editors Florie Borel Horae Gene Therapy Center University of Massachusetts Medical School Worcester, MA, USA
Christian Mueller Horae Gene Therapy Center University of Massachusetts Medical School Worcester, MA, USA Department of Pediatrics University of Massachusetts Medical School Worcester, MA, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7161-9 ISBN 978-1-4939-7163-3 (eBook) DOI 10.1007/978-1-4939-7163-3 Library of Congress Control Number: 2017943681 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Why Did We Write This Book? The book was triggered by the expansion in the field of alpha-1 antitrypsin (AAT) research. While by no means exhaustive, this book is meant to provide the essential protocols to laboratories ranging from new scientists in the field to more established alpha-one researchers. The aim of this book is to present a solid background by expert
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