Cadmium-tolerant endophytic Pseudomonas rhodesiae strains isolated from Typha latifolia modify the root architecture of
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ENVIRONMENTAL MICROBIOLOGY - RESEARCH PAPER
Cadmium-tolerant endophytic Pseudomonas rhodesiae strains isolated from Typha latifolia modify the root architecture of Arabidopsis thaliana Col-0 in presence and absence of Cd Gisela Adelina Rolón-Cárdenas 1,2 & Jackeline Lizzeta Arvizu-Gómez 3 & Juan Ramiro Pacheco-Aguilar 4 & Juan Vázquez-Martínez 5 & Alejandro Hernández-Morales 1,2 Received: 21 July 2020 / Accepted: 19 November 2020 # Sociedade Brasileira de Microbiologia 2020
Abstract In this work, we isolated four Cd-tolerant endophytic bacteria from Typha latifolia roots that grow at a Cd-contaminated site. Bacterial isolates GRC065, GRC066, GRC093, and GRC140 were identified as Pseudomonas rhodesiae. These bacterial isolates tolerate cadmium and have abilities for phosphate solubilization, siderophore production, indole acetic acid (IAA) synthesis, and ACC deaminase activity, suggesting that they are plant growth-promoting rhizobacteria. Bacterial inoculation in Arabidopsis thaliana seedlings showed that P. rhodesiae strains increase total fresh weight and number of lateral roots concerning non-inoculated plants. These results indicated that P. rhodesiae strains promote A. thaliana seedlings growth by modifying the root system. On the other hand, in A. thaliana seedlings exposed to 2.5 mg/l of Cd, P. rhodesiae strains increased the number and density of lateral roots concerning non-inoculated plants, indicating that they modify the root architecture of A. thaliana seedlings exposed to cadmium. The results showed that P. rhodesiae strains promote the development of lateral roots in A. thaliana seedlings cultivated in both conditions, with and without cadmium. These results suggest that P. rhodesiae strains could exert a similar role inside the roots of T. latifolia that grow in the Cd-contaminated environment. Keywords Endophytes . Plant growth-promoting rhizobacteria . Pseudomonas rhodesiae . Cadmium tolerance . Typha latifolia
Introduction Responsible Editor: Ieda Carvalho Mendes * Alejandro Hernández-Morales [email protected] 1
Posgrado en Ciencias Químicas, Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, San Luis Potosí, San Luis Potosí, Mexico
2
Facultad de Estudios Profesionales Zona Huasteca, Universidad Autónoma de San Luis Potosí, Romualdo del Campo 501, Fraccionamiento Rafael Curiel, CP 79060 Ciudad Valles, San Luis Potosí, Mexico
3
Secretaría de Investigación y Posgrado, Centro Nayarita de Innovación y Transferencia de Tecnología (CENITT), Universidad Autónoma de Nayarit, Tepic, Nayarit, Mexico
4
Facultad de Química, Universidad Autónoma de Querétaro, Querétaro, Querétaro, Mexico
5
Departamento de Ingeniería Bioquímica, Tecnológico Nacional de México (TecNM), Instituto Tecnológico Superior de Irapuato, Irapuato, Guanajuato, Mexico
Typha genus is commonly known as cattail and includes nine species, T. minima, T. elephantine, T. angustifolia, T. domingensis, T. capensis, T. latifolia, T. shuttleworthii, T. orientalis, and T. laxmannii, widely distributed thro
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