Pseudomonas laoshanensis sp. nov., isolated from peanut field soil

  • PDF / 850,531 Bytes
  • 6 Pages / 595.276 x 790.866 pts Page_size
  • 11 Downloads / 209 Views

DOWNLOAD

REPORT


ORIGINAL PAPER

Pseudomonas laoshanensis sp. nov., isolated from peanut field soil Ming‑Qing Wang1 · Chu‑Shu Zhang1,2 · Li‑Na Yu1 · Wei‑Qiang Yang1 · Kun Jiao1 · Kui‑Jie Gong3 · Xiao‑Yuan Chi1 · Jie Bi1 · Yu Song1 · Qing‑Li Yang4 · Jian‑Cheng Zhang1 · Gui‑Wen Yang5 · Jie Sun1  Received: 12 June 2020 / Revised: 16 September 2020 / Accepted: 30 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020

Abstract A novel Gram-stain-negative, aerobic strain, designated ­Y22T, was isolated from peanut field soil in Laoshan Mountain in China. Cells of strain Y ­ 22T were rod-shaped and motile by a single flagellum. The strain was found to be oxidase- and catalase-positive. 16S rRNA gene sequence based on phylogenetic analysis indicated that strain Y ­ 22T belonged to the genus Pseudomonas, and showed the highest 16S rRNA gene sequence similarity of 99.0% to Pseudomonas pelagia JCM ­15562T, followed by Pseudomonas salina JCM 1­ 9469T (98.4%), Pseudomonas sabulinigri JCM 1­ 4963T (97.9%), Pseudomonas bauzanensis CGMCC 1.9095T (97.6%) and Pseudomonas litoralis ­KCTC23093T (97.5%). The phylogenetic analysis based on multilocus sequence analyses with concatenated 16S rRNA, gyrB, rpoD and rpoB genes indicated that strain ­Y22T belonged to Pseudomonas pertucinogena lineage. The average nucleotide identity scores between strain ­Y22T and closely related species were 74.6–82.8%, and the Genome-to-Genome Distance Calculator scores were 16.4–44.9%. The predominant cellular fatty acids of strain Y ­ 22T were ­C18:1ω7c (29.6%), ­C17:0 cyclo (17.5%) and summed feature 3 ­(C16:1ω7c and/or C ­ 16:1ω6c) (17.4%). The genomic DNA G+C content was 57.9 mol%. On the basis of phenotypic characteristics, phylogenetic analyses and in silico DNA–DNA relatedness, a novel species, Pseudomonas laoshanensis sp. nov. is proposed. The type strain is ­Y22T (= JCM ­32580T = KCTC ­62385T = CGMCC 1.16552T). Keywords  Pseudomonas laoshanensis sp. nov · Bacteria · Taxonomy · Soil

Introduction The bacteria of the genus Pseudomonas belong to the Gammaproteobacteria and are aerobic, motile, Gram-negative rods (Hwang et al. 2009; Zhang et al. 2011). Members of this genus Pseudomonas are widely distributed in a variety Communicated by Erko stackebrandt. The GenBank/EMBL/DDBJ accession numbers for the partial 16S rRNA, gyrB, rpoB and rpoD gene sequences of strain ­Y22T are MG589919, MH587682, MH587681 and MT321511, respectively. The genome has been deposited in GenBank under the accession number of QOVF00000000 for stain ­Y22T. Electronic supplementary material  The online version of this article (https​://doi.org/10.1007/s0020​3-020-02067​-8) contains supplementary material, which is available to authorized users. * Jian‑Cheng Zhang jianch‑[email protected] * Jie Sun [email protected]

of natural sources, including soil, water, air, plants, seawater and clinical environment (Akita et al. 2019; Vasconcellos et al. 2017; Zhong et al. 2015; Sun et al. 2018; Kim et al. 2009; Timilsina et al. 2018; Zou et al. 2019; Wang et al. 2019; Ramírez-Bahena et al. 2