Pseudomonas laoshanensis sp. nov., isolated from peanut field soil
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ORIGINAL PAPER
Pseudomonas laoshanensis sp. nov., isolated from peanut field soil Ming‑Qing Wang1 · Chu‑Shu Zhang1,2 · Li‑Na Yu1 · Wei‑Qiang Yang1 · Kun Jiao1 · Kui‑Jie Gong3 · Xiao‑Yuan Chi1 · Jie Bi1 · Yu Song1 · Qing‑Li Yang4 · Jian‑Cheng Zhang1 · Gui‑Wen Yang5 · Jie Sun1 Received: 12 June 2020 / Revised: 16 September 2020 / Accepted: 30 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A novel Gram-stain-negative, aerobic strain, designated Y22T, was isolated from peanut field soil in Laoshan Mountain in China. Cells of strain Y 22T were rod-shaped and motile by a single flagellum. The strain was found to be oxidase- and catalase-positive. 16S rRNA gene sequence based on phylogenetic analysis indicated that strain Y 22T belonged to the genus Pseudomonas, and showed the highest 16S rRNA gene sequence similarity of 99.0% to Pseudomonas pelagia JCM 15562T, followed by Pseudomonas salina JCM 1 9469T (98.4%), Pseudomonas sabulinigri JCM 1 4963T (97.9%), Pseudomonas bauzanensis CGMCC 1.9095T (97.6%) and Pseudomonas litoralis KCTC23093T (97.5%). The phylogenetic analysis based on multilocus sequence analyses with concatenated 16S rRNA, gyrB, rpoD and rpoB genes indicated that strain Y22T belonged to Pseudomonas pertucinogena lineage. The average nucleotide identity scores between strain Y22T and closely related species were 74.6–82.8%, and the Genome-to-Genome Distance Calculator scores were 16.4–44.9%. The predominant cellular fatty acids of strain Y 22T were C18:1ω7c (29.6%), C17:0 cyclo (17.5%) and summed feature 3 (C16:1ω7c and/or C 16:1ω6c) (17.4%). The genomic DNA G+C content was 57.9 mol%. On the basis of phenotypic characteristics, phylogenetic analyses and in silico DNA–DNA relatedness, a novel species, Pseudomonas laoshanensis sp. nov. is proposed. The type strain is Y22T (= JCM 32580T = KCTC 62385T = CGMCC 1.16552T). Keywords Pseudomonas laoshanensis sp. nov · Bacteria · Taxonomy · Soil
Introduction The bacteria of the genus Pseudomonas belong to the Gammaproteobacteria and are aerobic, motile, Gram-negative rods (Hwang et al. 2009; Zhang et al. 2011). Members of this genus Pseudomonas are widely distributed in a variety Communicated by Erko stackebrandt. The GenBank/EMBL/DDBJ accession numbers for the partial 16S rRNA, gyrB, rpoB and rpoD gene sequences of strain Y22T are MG589919, MH587682, MH587681 and MT321511, respectively. The genome has been deposited in GenBank under the accession number of QOVF00000000 for stain Y22T. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02067-8) contains supplementary material, which is available to authorized users. * Jian‑Cheng Zhang jianch‑[email protected] * Jie Sun [email protected]
of natural sources, including soil, water, air, plants, seawater and clinical environment (Akita et al. 2019; Vasconcellos et al. 2017; Zhong et al. 2015; Sun et al. 2018; Kim et al. 2009; Timilsina et al. 2018; Zou et al. 2019; Wang et al. 2019; Ramírez-Bahena et al. 2
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