Metagenomics Methods and Protocols
This second edition explores up-to-date tools in various function-based technologies currently used in metagenomics. The chapters in this book discuss all of the working steps involved in these technologies, such as: DNA isolation from soils and marine sa
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Wolfgang R. Streit Rolf Daniel Editors
Metagenomics Methods and Protocols Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Metagenomics Methods and Protocols Second Edition
Edited by
Wolfgang R. Streit Mikrobiologie & Biotechnologie, Biocenter Klein Flottbek, University of Hamburg, Hamburg, Germany
Rolf Daniel Göttingen Genomics Laboratory, Department of Genomic and Applied Microbiology, Institut für Mikrobiologie und Genetik, Georg-August-Universität Göttingen, Göttingen, Germany
Editors Wolfgang R. Streit Mikrobiologie & Biotechnologie Biocenter Klein Flottbek University of Hamburg Hamburg, Germany
Rolf Daniel Göttingen Genomics Laboratory Department of Genomic and Applied Microbiology Institut für Mikrobiologie und Genetik Georg-August-Universität Göttingen Göttingen, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6689-9 ISBN 978-1-4939-6691-2 (eBook) DOI 10.1007/978-1-4939-6691-2 Library of Congress Control Number: 2016956093 © Springer Science+Business Media LLC 2010, 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Metagenomics is a key technology to the DNA-based exploration of the genomic potential from not-yet-cultivated microbes for ecology and biotechnology. Since the term “metagenome” was coined almost two decades ago, metagenomics has dramatically changed our view on many research areas such as microbial ecology, community biology, and microbiome research, and it has resulted in the rapid identification of many novel biomolecules with potential value to bio-based industrial process
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