Neuroproteomics Methods and Protocols

This new edition provides updated and novel protocols of neuroproteomics methods that encompass both global-scale as well as targeted and specialized topics, which are timely additions for the molecular and phenotypic analysis of the central nervous syste

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Firas H. Kobeissy Stanley M. Stevens, Jr. Editors

Neuroproteomics Methods and Protocols Second Edition

Methods

in

Molecular Biology

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Neuroproteomics Methods and Protocols Second Edition

Edited by

Firas H. Kobeissy Department of Psychiatry, University of Florida McKnight Brain Institute, Gainesville, FL, USA

Stanley M. Stevens, Jr. Department of Cell Biology, Microbiology, & Molecular Biology, University of South Florida, Tampa, FL, USA

Editors Firas H. Kobeissy Department of Psychiatry University of Florida McKnight Brain Institute Gainesville, FL, USA

Stanley M. Stevens, Jr. Department of Cell Biology,   Microbiology, & Molecular Biology University of South Florida Tampa, FL, USA

ISSN 1064-3745     ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6950-0    ISBN 978-1-4939-6952-4 (eBook) DOI 10.1007/978-1-4939-6952-4 Library of Congress Control Number: 2017935482 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Cover illustration: Painting: “Art of expression”, 2016. Acrylic on canvas 30x40 inches. American University of Beirut, Located at Dr. Elie El-Chaer Office. By the artist Iman Karout, MSc, Email: [email protected]. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.

Dedication To my brother Rabih Moshourab, and to those who have faith in science. Firas H. Kobeissy To the memory of my mother, Mary E. Stevens, and sister, Donna Stevens Miraoui. Stanley M. Stevens, Jr.

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Foreword I Major health care challenges r