CC-NBS-LRR, a set of VvCRP markers, can distinguish cultivars with ripe rot resistance to Colletotrichum pathogens in gr
- PDF / 1,920,956 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 59 Downloads / 173 Views
RESEARCH REPORT
CC-NBS-LRR, a set of VvCRP markers, can distinguish cultivars with ripe rot resistance to Colletotrichum pathogens in grapevine Hyun A. Jang1 · May Moe Oo1 · Dae‑Gyu Kim1 · Ha‑Yeon Yoon1 · Mi‑Reu Kim1 · Kyo‑Sang Lee1 · Khaing Shwe Zin Thinn1 · Saima Arif1 · Jia Guo Geng1 · Jiyoung Min1 · Dong Jun Lim2 · Youn Young Hur2 · Sang‑Keun Oh1 Received: 5 August 2020 / Revised: 10 August 2020 / Accepted: 13 August 2020 / Published online: 7 September 2020 © Korean Society for Horticultural Science 2020
Abstract Grape ripe rot is a fungal disease, which is almost inextinguishable particularly before and after harvest in most vineyards. The management of this disease largely depends on the breeding of resistant varieties. Thus, the search for resistant varieties should parallel the molecular analysis of the markers associated with disease resistance. Here, we tested the leaf inoculation assay of Colletotrichum acutatum and C. gloeosporioides by inoculating them into 350 grapevine varieties. As a result of the phenotypes common to both species, 119 varieties (34%) were classified as resistant. Consequently, we discovered the locus with nucleotide-binding sites and carboxyterminal leucine-rich repeat regions (NBS-LRR) associated with grape ripe rot resistance through genotyping-by-sequencing and genome-wide association study. Ultimately, we identified the Vitis vinifera Colletotrichum resistance protein (VvCRP) markers from chromosome 3, which belongs to the CC-NBS-LRR type, as disease-resistant proteins. These markers were verified as the melting peak, and their chromatograms of nucleotide sequences were visualized in each single nucleotide polymorphism (SNP) loci. All the SNP markers differentiated successfully the resistant from the susceptible cultivars. Interestingly, we also found that a resistant cultivar named Bailey Alicante A has a heterozygous genotype, substantiating its potential usefulness for crops such as grapevine with many hybrids. Altogether, our results indicate that VvCRP markers can be utilized to distinguish grape ripe rot-resistant cultivars, particularly in crossbreeding populations. Keywords CC-NBS-LRR · Colletotrichum spp. · Genome-wide association study · Genotyping-by-sequencing · Grape ripe rot resistance · VvCRP markers
1 Introduction
Communicated by Heakeun Yun, Ph.D. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13580-020-00290-2) contains supplementary material, which is available to authorized users. * Sang‑Keun Oh [email protected] 1
Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Republic of Korea
National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, Republic of Korea
2
With the advent of next-generation sequencing (NGS) technology, the use of molecular markers has become increasingly important in breeding agricultural crops. There are various types of molecular markers, such as randomly ampli
Data Loading...
