Characterization and fine mapping of a leaf yellowing mutant in common wheat
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ORIGINAL PAPER
Characterization and fine mapping of a leaf yellowing mutant in common wheat Heng Zhang1 · Yiming Chen1 · Ying Niu1 · Xu Zhang1 · Jia Zhao1 · Li Sun1 · Haiyan Wang1 · Jin Xiao1 · Xiue Wang1 Received: 15 April 2020 / Accepted: 29 May 2020 © Springer Nature B.V. 2020
Abstract Creation of leaf color mutants is the options in the study of photosynthesis, chloroplast development and Chlorophyll biosynthesis. In this study, a leaf color mutant NAU31 was identified from tissue culture of common wheat. In general, the mutant exhibited whole plant yellowing, fewer tillering, dwarfing and interestingly, improved resistance to wheat powdery mildew. Photosynthetic physiological analysis showed that the chloroplast inner structure varied in the mesophyll cells of the mutant, and this may lead to reduced chlorophyll content (chlorophyll “a”, Chlorophyll “b”, total chlorophyll and carotenoid), as well as decreased maximum quantum efficiency of photosystem II (Fv/Fm) and net photosynthetic rate. Transcriptome analysis indicated that the impairing of photosynthesis may be responsible for plant yellowing of NAU31. Inheritance analysis showed that NAU31 was a gain-of-function mutant and the yellowing trait was controlled by a dominant gene, namely Taymg (yellow mutant gene). SSR markers analysis preliminarily mapped Taymg to the distal short arm of chromosome 2D using an F2 population from the cross between NAU31 and W7984, a synthesized wheat. The mapping result was confirmed by BSA using a 55 K wheat SNP array. Through recombinants screening, the Taymg was finely mapped to the terminal region of 2DS, flanked by markers ZH47 and gwm296 and corresponding to a physical interval of 0.76 Mb. The cloning of Taymg will facilitate the dissection of the mechanism involved in chloroplast development of wheat and the coordinating of plant development and disease resistance. Keywords Wheat · Leaf color · Photosynthesis · Fine mapping Abbreviations BSA Bulked segregant analysis Car Carotenoid Chl Chlorophyll Chl a Chlorophyll “a” Chl b Chlorophyll “b” Chl a + b Chlorophyll “a” and Chlorophyll “b” Chl-Index Chlorophyll-Index Fv/Fm Maximum quantum efficiency PSII Photosystem II SNP Single nucleotide polymorphism
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10725-020-00633-0) contains supplementary material, which is available to authorized users. * Xiue Wang [email protected] 1
State Key Lab of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing 210095, Jiangsu, China
Introduction Photosynthesis is the primary energy for biosphere, and is the foundation for life on earth (Flood et al. 2016). More than 90% of crop biomass is derived from photosynthetic products, and the improvement of photosynthesis would undoubtedly increase crop yield (Makino 2011). Photosynthesis and translocation in relation to crop yield have been reviewed previously (Nasyrov 1978). There is a positive correlation between pho
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