Characterization and functional analysis of Krtap11-1 during hair follicle development in Angora rabbits ( Oryctolagus c
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Genes & Genomics https://doi.org/10.1007/s13258-020-00995-9
RESEARCH ARTICLE
Characterization and functional analysis of Krtap11‑1 during hair follicle development in Angora rabbits (Oryctolagus cuniculus) Shuang Liang1 · Zhiyuan Bao1 · Bohao Zhao1 · Tong Zhou1 · Jiali Li1 · Ming Liu1 · Shuaishuai Hu1 · Naisu Yang1 · Yang Chen1,2 · Xinsheng Wu1,2 Received: 29 April 2020 / Accepted: 7 September 2020 © The Genetics Society of Korea 2020
Abstract Background Keratin-associated protein (KAP), the structural protein molecule of hair fibers, plays a key role in determining the physical properties of hair. Studies of Krtap11-1 have focused only on its localization. Functional studies of Krtap11-1 in hair follicle development have so far not been reported. Objective This study aimed to provide evidence for the role of Krtap11-1 in skin and hair development. Methods Full-length cloning and analysis of Krtap11-1 were conducted to ascertain its function. Overexpression vectors and interference sequences were constructed and transfected into RAB-9 cells. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to investigate the hair follicle developmental stage of Krtap11-1, the expression of different tissues, and the effects on other hair follicle development-related genes. Results The full length of cloned Krtap11-1 was 947 bp. Krtap11-1 was confirmed to be a hydrophilic protein localized mostly in mitochondria. The greatest mRNA expression was observed in skin. Using a follicle synchronization model, it was found that Krtap11-1 mRNA expression levels first increased then decreased over the passage of time, principally during hair follicle catagen and telogen. Following the overexpression of Krtap11-1, mRNA expression levels of the WNT-2, KRT17, BMP-2, and TGF-β-1 genes increased, and LEF-1 decreased (P
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