The microfollicle: a model of the human hair follicle for in vitro studies
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The microfollicle: a model of the human hair follicle for in vitro studies Beren Ataç 1,2 & Flora Marta Kiss 1 & Tobias Lam 1,2 & Beatrix Fauler 3 & Clemens Edler 3 & Ping Hu 4 & Thi Phuong Tao 1 & Marian Jädicke 1 & Isabel Rütschle 1 & Reza P. Azar 5 & Scott Youngquist 4 & Thorsten Mielke 3 & Uwe Marx 1 & Roland Lauster 2 & Gerd Lindner 6 & Teresa DiColandrea 4 Received: 10 April 2020 / Accepted: 17 September 2020 / Editor: Tetsuji Okamoto # The Society for In Vitro Biology 2020
Abstract Access to complex in vitro models that recapitulate the unique markers and cell-cell interactions of the hair follicle is rather limited. Creation of scalable, affordable, and relevant in vitro systems which can provide predictive screens of cosmetic ingredients and therapeutic actives for hair health would be highly valued. In this study, we explore the features of the microfollicle, a human hair follicle organoid model based on the spatio-temporally defined co-culture of primary cells. The microfollicle provides a 3D differentiation platform for outer root sheath keratinocytes, dermal papilla fibroblasts, and melanocytes, via epidermalmesenchymal-neuroectodermal cross-talk. For assay applications, microfollicle cultures were adapted to 96-well plates suitable for medium-throughput testing up to 21 days, and characterized for their spatial and lineage markers. The microfollicles showed hair-specific keratin expression in both early and late stages of cultivation. The gene expression profile of microfollicles was also compared with human clinical biopsy samples in response to the benchmark hair-growth compound, minoxidil. The gene expression changes in microfollicles showed up to 75% overlap with the corresponding gene expression signature observed in the clinical study. Based on our results, the cultivation of the microfollicle appears to be a practical tool for generating testable insights for hair follicle development and offers a complex model for pre-clinical substance testing. Keywords Tissue engineering . Hair follicle . 3D model . In vitro . Organoid . Minoxidil
Introduction Hair follicle models can be leveraged to support the identification of therapeutic approaches to improve hair growth and Gerd Lindner and Teresa DiColandrea jointly supervised this work. Supplementary Information The online version of this article (https:// doi.org/10.1007/s11626-020-00513-x) contains supplementary material, which is available to authorized users. * Gerd Lindner [email protected] 1
TissUse GmbH, Berlin, Germany
2
Institute of Biotechnology, Department of Medical Biotechnology, Technische Universität Berlin, Berlin, Germany
3
Max Planck Institute for Molecular Genetics, Berlin, Germany
4
The Procter & Gamble Company, Mason Business Center, Cincinnati, OH 45040, USA
5
Centre for Modern Hair Transplantation, Berlin, Germany
6
provio GmbH, Glienicke/Nordbahn, Germany
appearance by providing insights into the molecular changes associated with disease and health. Advances in understanding human hair follicle function in he
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