Comparative Evaluation of Cytotoxicity and Phytochemical Composition of Centaurea iconiensis ( Rhaponticoides iconiensis
- PDF / 1,400,397 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 114 Downloads / 170 Views
(0123456789().,-volV) (0123456789().,-volV)
RESEARCH PAPER
Comparative Evaluation of Cytotoxicity and Phytochemical Composition of Centaurea iconiensis (Rhaponticoides iconiensis) Osman Tugay1 • Leyla Pas¸ayeva2
•
Eren Demirpolat3 • Mustafa S¸ahin4
Received: 10 April 2020 / Accepted: 6 November 2020 Ó Shiraz University 2020
Abstract Cancer is a disorder that results in abnormal and irregular proliferation of cells in the organ or tissue of the body. In Turkey flora, there are many medicinal plants that have great therapeutic importance. Rhaponticoides iconiensis is endemic to Turkey. In the present study, the methanol extracts and sub-extracts of leafy stem and flowers of R. iconiensis were evaluated for cytotoxic activity against Colo 205, A549, HepG2, MCF7 and Beas-2b cell lines by MTT method. The compounds of R. iconiensis extracts were characterized and quantitated by LC–MS/MS. As a result, methanol extracts were not cytotoxic against A549, but were cytotoxic to the Colo 205, HepG2, MCF-7 and Beas-2b cell lines. Among the sub-extracts, dichloromethane, ethyl acetate and n-hexane sub-extracts of flowers and dichloromethane sub-extract of the stem were most cytotoxic against A549, HepG2, MCF-7 and Colo 205 cell lines, respectively. As sub-extracts were most effective against MCF-7 cancer cell line, it can be said that extracts might be effective in estrogen-sensitive cancer cells such as breast and cervix. The cytotoxic effects of sub-extracts can be explained by the variances of compounds and synergistic effects of different secondary metabolites. Keywords Asteraceae Cytotoxicity Colo205 HepG2 MCF-7 LC–MS/MS
1 Introduction Cancer is the second widespread reason for death, and about 1 in 6 deaths is due to cancer in the world. According to the World Health Organization, about 9.6 million people are losing their lives owing to cancer every year over the world (Bray et al. 2018). It is known that there are some difficulties in the use of synthetic drugs in the treatment of cancer due to side effects and interactions. On the other hand, because of their wide application, therapeutic efficacy and low-toxicity natural products were described as & Leyla Pas¸ ayeva [email protected] 1
Department of Pharmaceutical Botany, Selc¸uk University, 42250 Konya, Turkey
2
Department of Pharmacognosy, Erciyes University, 38030 Kayseri, Turkey
3
Department of Pharmacology, Erciyes University, 38030 Kayseri, Turkey
4
Department of General Surgery, Selc¸uk University, 42250 Konya, Turkey
potent for the treatment of cancer and other various diseases. This effectiveness is linked to alone or synergistic effects of compounds present in extracts. However, the therapeutic efficacy of many plants has not been evaluated yet (Rufatto et al. 2013). The Asteraceae family (syn. Compositae) comprises several members that are used in folk medicine for the treatment of various diseases. Most of the recent studies were involved with the cytotoxicity of Asteraceae species and their secondary metabolites (Bakr et al.
Data Loading...
