Antioxidant activity, phytochemical composition of Andricus tomentosus and its antiproliferative effect on Mia-Paca2 cel
- PDF / 743,085 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 90 Downloads / 267 Views
ORIGINAL ARTICLE
Antioxidant activity, phytochemical composition of Andricus tomentosus and its antiproliferative effect on Mia-Paca2 cell line Özge Kılınçarslan Aksoy1 · Ramazan Mammadov2 · Mücahit Seçme3 Received: 17 June 2020 / Accepted: 7 September 2020 © Springer Nature B.V. 2020
Abstract Plant derived products are widely used in cancer treatment. Gall species has been preferred for treatment various diseases in folk medicine, but there are few studies on the anticancer effects of gall species. The present study reports the antioxidant activity and total secondary metabolites of extracts of A. tomentosus galls. The antioxidant potency of galls was carried out using different in-vitro model systems. Their cytotoxic efficacy on Mia-Paca cell line was also explored. Gall extract was found to contain a large amount of phenolic acids. The extract potently scavenged free radicals including DPPH ( IC50 9.56 ± 1.08 µg/mL), ABTS (IC50 18.51 ± 0.25 µg/mL). It can be seen as a potential source of antioxidants according to β-carotene/linoleic acid method (%92.58 ± 0.92) and Phosphomolybdenum assays (104.36 ± 4.95 mgAE/g). Gall extract also posses ability of metal chelating (%40.07 ± 2.30) and F e3+ (184.01 ± 2.83 mgTE/g) and C u2+ (89.81 ± 0.96 mgTE/g) reducing activity. According to total secondary metabolites tests, gall extract showed high total phenolic, total flavonoid and total tannin amount. HPLC analysis of the extract suggested it to contain caffeic acid (424.068.479 µg/g), ellagic acid (187.696.132 µg/g). XTT assay revealed gall extract to enhance percent survival of Mia-Paca2 cell line exposed A. tomentosus extracts. The best cytotoxic effect was determined in acetone extracts ( IC50: 124.7 µM). Expression of some genes (Bax, Bcl-2, FAS, BID, caspase-3, caspase-8, caspase-9, caspase-10, FADD, TRADD) in the apoptosis pathway was determined to invastigate apoptosis inducing activity. These results indicate that A. tomentosus galls possess potent antioxidant activity, when tested both in chemical as well as biological models. Keywords Andricus tomentosus · Galls · Antioxidant · XTT · HPLC
Introduction In recent years, the use of herbs for the prevention and treatment of cancer has gained more attention, due to various phytochemical components and fewer side effects. Cytotoxic potentials of four different medicinal plants against six different cancer cell lines (A-549, CCC-222, DU-145, MCF-7, K-562 and PC-3) and one normal cell line (Beas-2B) were investigated [1]. In another study was revealed that three
* Özge Kılınçarslan Aksoy [email protected] 1
Department of Biology, Science & Art Faculty, Pamukkale University, Denizli, Turkey
2
Department of Molecular Biology an Genetic, Muğla Sıtkı Koçman University, Mugla, Turkey
3
Department of Medical Biology, Medicine Faculty, TurkeyPamukkale University, Denizli, Turkey
different plant extracts have cytotoxic effect on MCF-7 cell line [2]. In one of the studies, phenolic compounds found in Ocimum basilicum and Thymus algeriensis essential
Data Loading...
