Comparative Transcriptome Analysis Profiles of Two Mulberry Varieties under Cadmium Stress
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Comparative Transcriptome Analysis Profiles of Two Mulberry Varieties under Cadmium Stress S. M. Jianga, *, R. Z. Huanga, Y. B. Jianga, and Z. B. Lia aThe
Sericultural Research Institute of Hunan Province, Changsha, 410127 China *e-mail: [email protected] Received November 2, 2019; revised March 19, 2020; accepted April 12, 2020
Abstract—This paper is try to investigate the molecular mechanisms of cadmium (Cd) response in mulberry (Morus alba L.) leaf. Transcriptome sequencing technology is used to analyze the profiles of low-Cd mulberry Zhehulusang (zhls) and high-Cd mulberry Xianmianzao (xmz) under Cd stress. Approximately 195 million clean reads were obtained, 2785 and 1211 differentially expressed genes (DEGs) were identified in zhls and xmz. The DEGs of zhls enriched in the pathway of flavonoid biosynthesis, photosynthesis, carbon fixation in photosynthetic organisms. The DEGs of xmz enriched in the pathway of flavonoid biosynthesis, plantpathogen interaction, phenylpropanoid biosynthesis. Through comparative analyses, the flavonoids may be the effective antioxidants conserved in mulberry under Cd stress. These information may be useful for further studies on investigating the molecular mechanisms of mulberry response to Cd stress. Keywords: Morus alba, cadmium, transcriptome, gene regulation DOI: 10.1134/S1021443720060096
INTRODUCTION Cd2+ is soluble in water, so it can be taken by plants. In recent years, accumulation of Cd in crop plants has become a major threat to agriculture and human health [1]. In this situation, the mulberry was found to have ecological functions. As a perennial woody tree, the mulberry could be used to remediate Cd polluted farmland soils owing to its large biomass and extensive root system [2]. 53.27–70.1% of Cd the mulberry accumulated was stored in the root and only about 10% were in the leaves. Lots of the Cd was located in the cell wall of the mulberry root and in soluble fraction of the mulberry leaf [3]. Plants can be distinguished in four groups in response to metal-induced stress [4]. Mulberry belongs to the metal-tolerant but non-hyperaccumulator species. Although it is known that the mulberry has tolerance to Cd stress, the changes of the metabolite regulatory networks of the mulberry response to the Cd stress is still unknown as plants possess a range of potential cellular mechanisms that may be involved in the detoxification of heavy metals. Integrated tranAbbreviation: Cd—cadmium; CDPKs—calcium-dependent protein kinases; CDF—cation diffusion facilitator; DEGs—differentially expressed genes; GO—gene ontology; GSH—glutathione; KEGG—Kyoto encyclopedia of genes and genomes; MAPKs—mitogen-activated protein kinase; MTs—metallothioneins; MTP—metal tolerance protein; NCBI—national center for biotechnology information; PCs—phytochelatins; PCS—phytochelatin synthase.
scriptome analyses can reveal many important metabolic pathways at the transcriptional level when the plants were under the abiotic stresses. As genome sequencing and analysis of the mulberr
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