Selective protein adsorption on highly hydrophilic surface modified with combined V-UV photon and plasma irradiation
- PDF / 293,086 Bytes
- 6 Pages / 612 x 792 pts (letter) Page_size
- 101 Downloads / 175 Views
0890-Y08-22.1
Selective protein adsorption on highly hydrophilic surface modified with combined V-UV photon and plasma irradiation Yuji Sato and Masataka Murahara Integrated Research Institute, Tokyo Institute of Technology P.O.Box I3-4 2-12-1 O-okayama Meguro-ku Tokyo, 152-8550, JAPAN ABSTRACT A highly hydrophilic surface, with a water contact angle smaller than 20 degrees, was rendered to a poly-methylmethacrylate [PMMA] by Xe2 excimer lamplight and plasma treatment. In result, the implant material that was highly biocompatible and adhesive to soft tissue was developed. The PMMA surface, which had been pretreated by ammonia plasma, was irradiated with Xe2 excimer lamplight in the presence of hydrazine as a reaction solution. In order to evaluate the wettability of the modified sample, the contact angle with water was measured. The contact angle was decreased from 83 degrees for untreated PMMA to 60 degrees when irradiated the Xe2 lamplight for 30 minutes. With the ammonia plasma pretreatment, the contact angle of the treated sample became to 7 degrees at the 1 minute Xe2 lamp irradiation. INTRODUCTION The PMMA, poly-ethyleneterephthalate [PET], and poly-tetrafluoroethylene [PTFE] are employed as a soft tissue implant material or cell culture substrate, for they have excellent characteristics of machinability and bioinertness. These polymers, however, have low adhesion to tissue because of their water repellency. Development of a biocompatible material that has an affinity to soft tissue is desired. In order to improve the biocompatibility, Imai and Watanabe [1] modified the wettability of an ethylene-vinyl alcohol copolymer film by soaking the sample in alcohol and benzene separately to enhance cell adsorption. After cultivating epithelial cells on the sample surface, they reported that the maximum adsorption of epithelial cell was found on the sample when the water contact angle was 60 to 70 degrees. Okada and Ikada reported that the carboxyl groups in poly (acrylic acid) chains were grafted onto the silicone surface, and the collagen was able to immobilize there [2]. On the other hand, M.C.L. Martins et al. produced a poly(hydroxyethylmethacrylate) (PHEMA) and a poly(hydroxyethylacrylate)(PHEA) film by the graft polymerization onto the poly(etherurethane)(PU) films to absorb the ALB and fibrinogen
0890-Y08-22.2
[3]. Hegemann et al. [4] demonstrated plasma etching in Ar, O2, CF4 and SF4 atmospheres. As for ion beam irradiation, Suzuki et al. [5] reported on protein adsorption onto PTFE, in which collagen was adsorbed on the PTFE treated by helium ion irradiation. Davenas et al. [6] incorporated oxygen groups on polyethylene, polystyrene and PMMA by N+ and He+ ion beam irradiations, and Calcagno et al. [7] defluorinated PTFE by He+ ion beam irradiation. Lee et al. formed the micropatterning on the oxygen plasma treated polystyrene surface by using KrF excimer laser ablation method and cultivated the NG 108-15 neuroblastoma x glioma hybrid cells [8]. They reported qualitatively that the protein adsorption depen
Data Loading...
