Comparison of spherical and rod-like morphologies of SBA-15 for enzyme immobilization
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Comparison of spherical and rod‑like morphologies of SBA‑15 for enzyme immobilization Sarawut Kingchok1,2 · Soraya Pornsuwan1
© Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Mesoporous silica, namely SBA-15, has been used for enzyme immobilization to help improve thermal stability of the enzymes and, in some cases, increase the enzymatic activity. It has been shown that morphology of SBA-15 is one of the factors influencing kinetics of enzyme adsorption. We reported, here, the adsorption kinetics and peroxidase activity of cytochrome c (cytc) in spherical SBA-15 in comparison of three different rod-like (fibrous, fiber, and rope) SBA-15 samples. The experimental adsorption profile fitted much better to the pseudo-second order than the pseudo-first order model. The maximum loading capacity of cytc was 466, 423, 390, and 352 mg/g for fibrous, rope, fiber, and spherical SBA-15, respectively. The rate constant for cytc adsorption for spherical SBA-15 was 3 times slower than rope and fiber SBA-15, and 15 times slower than fibrous SBA-15. The activity of cytc after immobilization in SBA-15 samples depended on the amount of cytc loading. The cytc loading in all SBA-15 at lower than 10 µmol/g showed higher activity than in free cytc in solution. The maximum activity of immobilized cytc was found in spherical SBA-15 at cytc loading of 1.00 µmol/g (~ 7 times higher than in free cytc). Finally, the Fe(III) at the heme group of cytc/SBA-15 samples with high activity was examined to contain high-spin state. The investigation in this work could suggest useful information for the preparation of SBA-15 for enzyme immobilization to appropriate applications, such as drug delivery. Keywords Mesoporous material · Morphologies · SBA-15 · Immobilization of cytochrome c · Peroxidase activity
1 Introduction Mesoporous silicas (MPS) are promising materials as solid supports for enzyme immobilization that can be utilized in many applications, for instance, biomolecular separation, biocatalysis, biosensors, and drug delivery [1–3]. In general, MPS, specifically, MCM-41, and SBA-15, is prepared by the self-assembly of surfactants and silicates. The synthesis route provides MPS materials with unique properties Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10934-020-00932-x) contains supplementary material, which is available to authorized users. * Soraya Pornsuwan [email protected] 1
Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, 272 Rama 6 Road, Phayathai, Bangkok, Thailand
Present Address: School of Materials Science and Innovation, SC1 building, 999 Phutthamonthon Sai 4 Rd, Salaya, Phutthamonthon, Nakhon Pathom 73170, Thailand
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of high surface area (~ 1000 m 2/g) and pore volume, narrow pore size distribution, and high stability over a wide range of temperature [4, 5]. Using the standard hydrothermal method followed by calcination, MPS materials with tunable pore d
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