Comparison of the Effects of KE and AED Peptides on Functional Activity of Human Skin Fibroblasts during Their Replicati
- PDF / 342,944 Bytes
- 4 Pages / 594 x 792 pts Page_size
- 13 Downloads / 148 Views
Cell Technologies in Biology and Medicine, No. 3, November, 2020
Comparison of the Effects of KE and AED Peptides on Functional Activity of Human Skin Fibroblasts during Their Replicative Aging N. V. Fridman1, N. S. Linkova1,2, E. O. Kozhevnikova1, E. O. Gutop1, and V. Kh. Khavinson1
Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 3, pp. 197-201, September, 2020 Original article submitted March 12, 2020 We studied the effect of KE and AED peptides on the expression of sirtuin-1, sirtuin-6, collagen I, cytokines (IL-1, TGF-β), and transcription factor NF-κB in human skin fibroblasts during their replicative aging. Immunocytochemical analysis and confocal microscopy showed that KE peptide reduces the synthesis of factors of the inflammatory response IL-1, NF-κB, and TGF-β and stimulates the synthesis of sirtuin-6. KE peptide normalizes the immunological function of human skin fibroblasts during their aging. AED peptide activates the synthesis of sirtuin-1, sirtuin-6, and collagen I in human skin fibroblasts during their replicative aging, which attests to its geroprotective effect. Key Words: short peptides; replicative aging; human skin fibroblasts; sirtuins; collagen I Involutional changes in the human body begin at the cellular level, when the cells lose their functional activity, ability to proliferate, migrate, and synthesize tissue-specific proteins [9]. The most pronounced agerelated changes are observed in the skin cells. The skin is involved in barrier, excretory, immune, and receptor functions of the body as well as in thermoregulation and maintenance of the water-salt metabolism. Age-related skin changes are associated, among other things, with increasing deterioration of the intercellular substance of the dermis due to reduced synthesis of proteins of the intercellular matrix by fibroblasts, on the one hand, and activation of enzymes that destroy it, on the other. Fibroblasts are widely used in the study of repair mechanisms and the analysis of ontogenesis mechanisms in gerontology. They are the main type of dermis cells responsible for the synthesis and secretion of the main components of the dermal ma1 Department of Biogerontology, St. Petersburg Institute of Bioregulation and Gerontology, St. Petersburg, Russia; 2Department of Therapy, Geriatrics, and Anti-Aging Medicine, Academy of Postgraduate Education, Federal Research Clinical Centre of Federal Medical-Biological Agency of Russia, Moscow, Russia. Address for correspondence: [email protected]. N. S. Linkova
trix, such as collagen, elastin, and glycosaminoglycans [15]. Replicative aging of skin fibroblasts is primarily associated with a decrease in the synthesis of collagen, hyaluronic acid, and other bioactive substances [4]. Skin aging remains a pressing problem of modern gerontocosmetology. The imbalance between the synthesis of the intercellular matrix by fibroblasts and its degradation can be corrected by using peptide geroprotectors [1,3]. A promising methods of slowing down the process of age-related skin changes is th
Data Loading...
