The complete genome sequence of Bacillus velezensis LPL061, an exopolysaccharide-producing bacterium
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GENOME REPORTS
The complete genome sequence of Bacillus velezensis LPL061, an exopolysaccharide‑producing bacterium Ruiyun Wu1 · Yuxuan Qin3 · Qian Shen2 · Pinglan Li1 Received: 24 February 2020 / Accepted: 24 April 2020 © King Abdulaziz City for Science and Technology 2020
Abstract Bacillus velezensis LPL061, which shows strong exopolysaccharide (EPS) producing capacity, was isolated from carnations in Beijing, China. The complete genome of LPL061 comprised a single circular chromosome (3,907,268 bp; G+C content of 46.7%) with 3,737 coding DNA sequences, 26 rRNA, and 89 tRNA. According to genome analysis, 12 protein-coding genes which related to polysaccharide biosynthesis in LPL061 were identified. Comparative genome analysis revealed that the EPS biosynthetic gene cluster was relatively conserved among Bacillus species. EPS showed approximately 60% inhibitory activity on the α-glucosidase at 100 μg/mL. The results of quantitative reverse transcription PCR further demonstrated that compared to insulin-resistant model with insulin (500 μg/mL) (without EPS treatment), the insulin-resistant HepG2 cells treated with EPS decreased the expression of phosphoenolpyruvate carboxykinase (PEPCK) from 4.425 to 0.1587, glucose6-phosphatase (G6Pase) decreased from 4.272 to 0.1929, and glycogen synthase kinase3β (GSK(3)β) decreased from 2.451 to 0.993, respectively. Meanwhile, EPS treatment increased GS expression and resulted in intracellular glycogen concentration increased from 28.30% to 86.48%, which further supported that EPS form LPL061 could reduce the concentration of blood glucose effectively. These results could be beneficial for better understanding of the hypoglycemic mechanism of B. velezensis LPL061 EPS and developing an EPS-based anti-diabetic agent in the future. Keywords Bacillus velezensis LPL061 · Complete genome sequence · Exopolysaccharide · Hypoglycemic
Introduction Beneficial microorganisms can produce a variety of exopolysaccharides (EPS) that are safe for human beings. EPSs isolated from Bifidobacterium and Lactobacillus have immunomodulatory activity, thus protecting hosts against bacterial Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13205-020-02228-y) contains supplementary material, which is available to authorized users. * Pinglan Li [email protected] 1
Key Laboratory of Precision Nutrition and Food Quality, Key Laboratory of Functional Dairy, Ministry of Education; College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua East Road, East Campus, Haidian District, Beijing 100083, China
2
Department of Microbiology, Ohio State University, Columbus, USA
3
Department of Biology, Northeastern University, Boston, MA 02115, USA
and viral infections (Emanuele et al. 2016; Liu et al. 2011; Yu et al. 2019). Hypoglycemic is one of the most important biological functions of polysaccharides produced by lactic acid bacteria (Bajpai et al. 2016). Diabetes mellitus is a chronic metabolic disease c
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