Complete genome sequence of the aromatic-hydrocarbon-degrading bacterium Achromobacter xylosoxidans DN002
- PDF / 1,203,318 Bytes
- 5 Pages / 595.276 x 790.866 pts Page_size
- 74 Downloads / 228 Views
SHORT COMMUNICATION
Complete genome sequence of the aromatic‑hydrocarbon‑degrading bacterium Achromobacter xylosoxidans DN002 Yanbing Li1 · Yuexin Tian1 · Zhidan Hao1 · Yanling Ma1 Received: 7 April 2020 / Revised: 30 June 2020 / Accepted: 9 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Achromobacter xylosoxidans DN002 is capable of utilizing numerous aromatic hydrocarbons as sole carbon and energy resource. In this study, the whole genome of strain DN002 was sequenced and analyzed, which consisted of one circular chromosome of 5,943,204 bp and a 278,917 bp plasmid with an average GC content of 65.46 mol%, 5694 protein-coding genes, 13 rRNA genes and 57 tRNA genes. Analysis of cluster of orthologous group (COG) demonstrated that strain DN002 had remarkable gene abundance foramino acid transport and metabolism, transcription, inorganic ion transport and metabolism, energy production and conversion, and carbohydrate transport and metabolism. Genes related to biodegradation of aromatic hydrocarbons, chemotaxis and flagella were identified from the genome, which will advance our fundamental understanding the molecular mechanism for degradation and metabolizing of aromatic hydrocarbons. Keywords Achromobacter xylosoxidans DN002 · Genome sequence · Biodegradation · Aromatic hydrocarbons
Introduction Aromatic hydrocarbon pollution poses a severe threat to ecosystems due to the combustion of plant material, oil spills and the burning of petroleum derivatives (Fernandez et al. 2011; Guo et al. 2017). Complex ecosystems comprising various types of microorganisms are widespread in the environments to fight against those pollutants (Wackett 2012; Khalilova et al. 2014; Gao et al. 2015; He et al. 2018). These hydrocarbon-degrading strains include at least sixty genera of aerobic bacteria and five genera of anaerobic bacteria (Das and Chandran 2011; Singh et al. 2017; Brzeszcz and Kaszycki 2018; Lu et al. 2019). Achromobacter xylosoxidans
is the type species of the genus Achromobacter that has been reported to have capability to degrade aromatic hydrocarbons (Deng et al. 2014; Amin et al. 2015; Ma et al. 2015; Iyer and Damania 2016; Mendez et al. 2018). Among them, A. xylosoxidans DN002 that was isolated from petroleumcontaminated soils could degrade fluoranthene via dioxygenation at the C-2,3 position through formation phthalate and catechol as intermediates (Ma et al. 2015). To further explore the molecular mechanism of aromatic hydrocarbon degradation and advance the potential biotechnological applications of this strain, we present the complete genome sequence of strain DN002 and its features.
Data description Communicated by Erko Stackebrandt. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-01977-x) contains supplementary material, which is available to authorized users. * Yanling Ma [email protected] 1
Shaanxi Provincial Key Laboratory of Biotechnology, Key Laboratory of Resources Biology and Biotechnology in Wester
Data Loading...
