Molecular characterization of a novel fusarivirus infecting the edible fungus Auricularia heimuer
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Molecular characterization of a novel fusarivirus infecting the edible fungus Auricularia heimuer Xuefei Li1,2 · Kunpeng Sui1,2 · Jiatao Xie3 · Du Hai3 · Weiqi Yin1,2 · Frederick Leo Sossah1 · Daohong Jiang3 · Bing Song1 · Yu Li1 Received: 19 May 2020 / Accepted: 20 July 2020 © Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract Here, we describe a novel mycovirus, Auricularia heimuer fusarivirus 1 (AhFV1), isolated from the edible fungus Auricularia heimuer strain CCMJ1296. The virus has a single-stranded positive-sense [+ssRNA] genome of 7,127 nucleotides containing two overlapping open reading frames (ORFs) and a poly(A) tail. The large ORF1 encodes a polyprotein of 1,637 amino acids (aa) with conserved RNA-dependent RNA polymerase (RdRp) and DEAD-like helicase superfamily (DEXDc) domains. ORF2 encodes a putative 633-aa protein with unknown function. A BLAST search showed that the nucleotide sequence of the AhFV1 genome is 41.28% identical to that of Sclerotium rolfsii fusarivirus 2 and 40.49% identical to that of Sclerotium rolfsii fusarivirus 1. Phylogenetic analysis based on RdRp and helicase (Hel) sequences indicated that AhFV1 is related to unclassified mycoviruses and other fusariviruses. Our data suggest that AhFV1 should be classified as a member of the newly proposed family “Fusariviridae”. This is the second virus and the first full genome sequence of a fusarivirus from A. heimuer. Mycoviruses are common in all of the major fungi, and an increasing number of novel mycoviruses have been identified in recent years [1–3]. At present, most of the reported viral genomes are positive single-stranded RNA [+ssRNA] or double-stranded RNA (dsRNA) [2, 4–10]. A few fungal, viral genomes are negative single-stranded RNA [−ssRNA] or single-stranded DNA (ssDNA) [11–14]. Mycoviruses Handling Editor: Ioly Kotta-Loizou. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00705-020-04781-6) contains supplementary material, which is available to authorized users. * Bing Song [email protected] * Yu Li [email protected] 1
Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, Jilin, China
2
College of Plant Protection, Jilin Agricultural University, Changchun 130118, Jilin, China
3
State Key Laboratory of Agricultural Microbiology, Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan 430070, Hubei, China
with +ssRNA genomes are now divided into eight families: Alphaflexiviridae, Barnaviridae, Endornaviridae, Gammaflexiviridae, Hypoviridae, Narnaviridae, Deltaflexiviridae, and Botourmiaviridae [15–17]. The “Fusariviridae” are a recently proposed virus family. The genomes of viruses in this family are +ssRNA, with sizes of 6–10 kb [7]. Most fusariviruses infect their hosts without causing any morphological changes [6, 7]. Auricularia heimuer, an edible fungus, is popular with consumers due to its high nutritive, medi
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