Core collection construction and evaluation of the genetic structure of Glycyrrhiza in China using markers for genomic s
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RESEARCH ARTICLE
Core collection construction and evaluation of the genetic structure of Glycyrrhiza in China using markers for genomic simple sequence repeats Yaling Liu . Yaping Geng . Xiaodong Xie . Pengfei Zhang . Junling Hou . Wenquan Wang
Received: 4 September 2019 / Accepted: 20 April 2020 Ó Springer Nature B.V. 2020
Abstract Glycyrrhiza is a widely used traditional Chinese herb with medicinal values. In order to avoid handling a large number of collections with redundant genetic information, it is necessary to construct a core collection with minimal accessions and maximal genetic diversity. In this study, the initial collection consisted of 736 accessions from four species, each of which was analyzed using SSR markers. The advanced M (maximization) strategy of PowerCore was utilized to select 124 accessions for the core collection. The core collection retained 100% of the alleles of the initial collection, and there was no significant difference regarding the genetic diversity parameters compared to the initial collection. PCoA and AMOVA also showed that the core collection has
a similar genetic distribution. NJ and STRUCTURE analyses showed that the core collection can be divided into three groups: The first group is represented by the majority of Glycyrrhiza uralensis Fisch.; the second group is represented by Glycyrrhiza inflata Batalin and Glycyrrhiza glabra L.; and finally, the third group is represented by Glycyrrhiza pallidiflora Maxim. The results indicate that the core collection is an effective tool for representing the genetic diversity of the initial collection. The core collection also provides a useful primary resource to improve the conservation of genetic diversity and to improve Glycyrrhiza breeding. Keywords Glycyrrhiza Core collection SSR marker Genetic diversity
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10722-020-00944-1) contains supplementary material, which is available to authorized users. Y. Liu Y. Geng X. Xie P. Zhang College of Life Science, Shanxi Province, Shanxi Agricultural University, Taigu 030801, People’s Republic of China e-mail: [email protected]
J. Hou (&) W. Wang (&) School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, People’s Republic of China e-mail: [email protected]
Y. Geng e-mail: [email protected]
W. Wang e-mail: [email protected]
X. Xie e-mail: [email protected]
W. Wang Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, People’s Republic of China
P. Zhang e-mail: [email protected]
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Genet Resour Crop Evol
Introduction Licorice is a perennial leguminous plant belonging to the genus Glycyrrhiza. There are a total of eight Glycyrrhiza species distributed in China, but only three are certified genuine medicinal plants included in the Pharmacopoeia of the People’s Republic of China: Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Batalin and Gly
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