Cytotoxicity of Aluminum-Silica Matrices Modified with Carbon Nanotubes
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Bulletin of Experimental Biology and Medicine, Vol. 169, No. 5, September, 2020 BIOTECHNOLOGIES
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Cytotoxicity of Aluminum-Silica Matrices Modified with Carbon Nanotubes
A. P. Lykov, L. N. Rachkovskaya, O. V. Poveshchenko, M. A. Surovtseva, I. I. Kim, N. A. Bondarenko, E. E. Rachkovskii, M. A. Korolev, and A. Y. Letyagin Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 169, No. 5, pp. 619-622, May, 2020 Original article submitted January 22, 2020 We studied the effect of aluminum-silicon matrices modified with carbon nanotubes on proliferation and production of nitric oxide by splenocytes, thymocytes, and bone marrow mononuclear cells of female db/db mice. Synthesized matrices decreased cell proliferation and suppressed NO production by the studied cells. Key Words: aluminum; polymethylsiloxane; single-wall carbon nanotubes; proliferation; nitric oxide Carbon nanotubes are considered as a method of targeted drug delivery [4]. However, the safety of nanomaterials for animals and humans remains an open question. The safety of carbon nanotubes (no initiation of inflammation in the lungs and genotoxicity) was shown [3,5]; however, their genotoxic effects on human cells (aberration of fibroblast DNA) were reported [7]. Here we studied the influence of porous aluminum oxide/polymethylsiloxane matrices modified with single-walled carbon nanotubes on functional properties of splenocytes, thymocytes, and bone marrow mononuclear cells of db/db mice.
MATERIALS AND METHODS Porous material based on γ-aluminum oxide (γ-Al2O3, Katalizator) with particle size 0.1 mm and 0.2-0.6 mm with organosilica polymer polymethylsiloxane (PMS, molecular weight 18,000-19,000; Penta) with particle diameter up to 60 µ [1] immobilized on its surface was used as the carrier. Single-walled carbon nanotubes (SWCN, Tuball-99, OCSiAl) of high purity in the Research Institute of Clinical and Experimental Lymphology — Affiliated Branch of the Federal Research Center Institute of Cytology and Genetics, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia. Address for correspondence: [email protected]. A. P. Lykov
form of an aqueous dispersion of Tuball Batt H2O (0.4 wt% Tuball and 0.6 wt% Na-carboxymethyl cellulose as dispersant) were used as a modifier. Modification of the γ-Al2O3/PMS composition was carried out by immobilization of SWCN in the aqueous phase by physical adsorption. Then, the obtained matrices were dried and subjected to short-term low-temperature heating up to 120°С. The obtained matrices were loose powder materials of silver color with uniform distribution of SWCN on the particle surface. The experiments were performed on db/db mice (genetically determined type 2 diabetes mellitus) with strict adherence to the principles of humanity. Bone marrow mononuclear cells (BM-MNC) were isolated from the femoral bones. Spleen cells (splenocytes) and thymus cells (thymocytes) were obtained after homogenization of the spleen and thymus. BM-MNC, splenocytes, and thymocytes were resuspended in RPMI-16
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