Electrochemical determination of sulfamethazine using a gold electrode modified with multi-walled carbon nanotubes, grap
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ORIGINAL PAPER
Electrochemical determination of sulfamethazine using a gold electrode modified with multi-walled carbon nanotubes, graphene oxide nanoribbons and branched aptamers Baoshan He 1 & Ming Li 1 & Mannan Li 1 Received: 17 December 2019 / Accepted: 30 March 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract An aptasensor assay for sulfamethazine is introduced based on a gold electrode modified with multi-walled carbon nanotubes@graphene oxide nanoribbons (MWCNTs@GONRs) nanocomposites. The MWCNTs@GONRs nanocomposites were synthesized by a chemical longitudinal partially unzipping method. Its properties are characterized by scanning electron microscope (SEM), energy dispersive spectrometric analysis (EDS), transmission electron microscope (TEM) and X-ray diffraction (XRD). In the presence of sulfamethazine, the conformation of the aptamer changes and a G-quadruplex structure is formed. It inhibits the electron transfer of the redox probe [Fe(CN)6]3−/4− on the electrode surface, causing weakening of the peak current response at the peak potential of 0.17 V (vs. Ag/AgCl). Using differential pulse voltammetry, a linear response was obtained for sulfamethazine concentrations in the range 0.01 ~ 50 ng mL−1 with a detection limit of 5.2 pg mL−1. Keywords Aptamer . Au-S bond . Recognition element . [Fe(CN)6]3−/4- . Electrodeposited gold nanoparticles
Introduction Sulfamethazine (SM2), a kind of sulfonamides, plays an important role in preventing and treating livestock diseases [1]. The mechanism of action is that SM2 competes with paminobenzoic acid (PABA) with similar structure to bind dihydrofolate synthetase in bacteria, thus inhibiting the growth and breeding of bacteria [2]. However, the unreasonable use of SM2 has led to excessive residues in animalderived foods, which can be transmitted to the human body through the food chain [3]. It is harmful to human health. In order to ensure food safety, a variety of analytical methods have been developed for the determination of SM2, such as enzyme-linked immunoassay (ELISA) [4], high performance Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04244-4) contains supplementary material, which is available to authorized users. * Baoshan He [email protected] 1
School of Food Science and Technology, Henan University of Technology, Lianhua Road 100#, Zhengzhou High & New Technology Industries Development Zone, Zhengzhou 450001, Henan Province, People’s Republic of China
liquid chromatography (HPLC) [5], fluorescence immunoassay [3], mass spectrometry (MS) [6] and liquid chromatography tandem mass spectrometry (LC-MS) [7]. Antibody-based fluorescence immunoassay and ELISA have high specificity; however, they are often too expensive and unstable for practical applications. The instrumental analyses such as CE, HPLC and LC/MS could provide accurate determination of SM2. However, these methods are time-consuming and need large instrumentation and professional staff operation, which are difficu
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