Determination of thermal tolerance in rainbow trout Oncorhynchus mykiss based on effective time, and its reproducibility
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ORIGINAL ARTICLE Biology
Determination of thermal tolerance in rainbow trout Oncorhynchus mykiss based on effective time, and its reproducibility for a large number of fish Toshinao Ineno1,2 · Kazuya Yamada2 · Koichi Tamaki2 · Ryusuke Kodama2 · Engkong Tan3,4 · Shigeharu Kinoshita4 · Koji Muto5 · Takashi Yada5 · Shoji Kitamura5 · Shuichi Asakawa4 · Shugo Watabe4,6 Received: 2 April 2020 / Accepted: 12 June 2020 © Japanese Society of Fisheries Science 2020
Abstract Thermally selected male rainbow trout selectively bred at high temperatures were crossed with females of the Nikko strain with normal thermal tolerance. The F2 pedigrees, comprising 1641 individuals, were divided into 26 batches. The fish were acclimated to 17 °C, then their thermal tolerance determined by measuring the effective time (ET) required for them to lose equilibrium at an approximate upper lethal temperature (28 °C). The fish were categorized into 0- to
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