Development and characterization of polymorphic microsatellites in Ficus sarmentosa var. henryi
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MICROSATELLITE LETTERS
Development and characterization of polymorphic microsatellites in Ficus sarmentosa var. henryi Chang-Hong Yang • Yuan-Yuan Ding Xin Tong
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Received: 16 April 2014 / Accepted: 2 June 2014 Springer Science+Business Media Dordrecht 2014
Abstract Figs are keystone resources of tropical and subtropical forests since they are speciose and supporting plenteous animals. Ficus sarmentosa var. henryi (King) Corner, a functionally dioecious evergreen fig, is threatened by habitat loss and fragmentation. However, genetic information has hitherto been nearly terra incognita for this species, which hinders the protection and management of this species and the forests. In this study, we developed and characterized 10 polymorphic microsatellites in F. sarmentosa var. henryi. The numbers of alleles per locus varied from 2 to 15. The observed and expected heterozygosities within populations were 0–0.857 and 0–0.876, respectively. These polymorphic microsatellites will contribute to further population genetic studies, and thereby benefit resource conservation and forest protection. Keywords Ficus sarmentosa var. henryi Moraceae Microsatellite Genetic diversity Tropical and subtropical forests harbor a large part of biodiversity on the Earth, yet facing a crisis of rapid decline due to anthropogenic disturbances. Ficus (Moraceae), one of the most speciose genera of angiosperms, plays a prominent role in these hyper-diverse forests. Not
Electronic supplementary material The online version of this article (doi:10.1007/s12686-014-0238-0) contains supplementary material, which is available to authorized users. C.-H. Yang Y.-Y. Ding X. Tong (&) School of Ecology and Environmental Sciences, Tiantong National Station for Forest Ecosystem, Shanghai Key Lab of Urban Ecological Processes and Eco-Restoration, East China Normal University, Dongchuan Road 500, Shanghai 200241, China e-mail: [email protected]
only figs per se, but also the invertebrates and vertebrates supported by figs make substantial contribution to these forest ecosystems (Harrison 2005). The obligate mutualism between figs and their specific pollinating wasps (Chen et al. 2012), for example, is an important among-trophic interaction in natural ecosystems. Ficus sarmentosa var. henryi (King) Corner, a functionally dioecious evergreen fig, is a keystone resource of many tropical and subtropical forests, but threatened by habitat loss and fragmentation. So far, lacking information on genetic diversity and structure impedes protecting this species. For this purpose, we isolated and characterized microsatellites in F. sarmentosa var. henryi. Total genomic DNA was extracted from silica gel-dried leaves of F. sarmentosa var. henryi with the use of the Plant Genomic DNA Kit (Tiangen, Beijing, China). Following Tong et al. (2012), we used the biotin-streptavidin capture method to develop microsatellite primers. After digested with the restriction enzyme MseI (New England Biolabs, Beverly, Massachusetts, USA), genomic DNA (250 ng) was linked to an
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