Development and characterization of twelve novel polymorphic microsatellite loci in the Percocypris pingi
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MICROSATELLITE LETTERS
Development and characterization of twelve novel polymorphic microsatellite loci in the Percocypris pingi Weitao Li • Lei Pan • Zhiqing Ren • Yanfu Que • Meihua Xiong • Xiaolin Liao • Nian Xu • Dongmei Xu • Ke Shao • Bin Zhu
Received: 31 December 2013 / Accepted: 4 January 2014 Ó Springer Science+Business Media Dordrecht 2014
Abstract Percocypris pingi is an endemic subspecies of Percocypris mainly distributed in upper Yangtze river (Jinsha river) and its branch river. This species has been listed as a near threatened species in IUCN Red List. Twelve microsatellite markers were developed and the allele number of these polymorphic markers ranged from 2 to 5. Observed and expected heterozygosity in the population ranged from 0.381 to 0.952 and from 0.517 to 0.787, respectively. No significant linkage disequilibrium was detected among the loci. No loci significantly (P \ 0.004) deviated from Hardy–Weinberg equilibrium after Bonferroni correction. These microsatellite markers will provide helpful tools not only for genetic analyses of wild P. pingi but also for possible re-stocking management in the long turn. Keywords Percocypris pingi Endemic fish Microsatellite loci Genetic structure
The species of Percocypris (Cyprinidae, Teleostei) which consist of three subspecies are fierce predatory freshwater fishes inhabiting large rivers or lakes, in southwestern China and northern Vietnam. Percocypris pingi, an endemic subspecies of Percocypris mainly distributed in upper
Weitao Li and Lei Pan have contributed equally to this work. W. Li L. Pan Z. Ren Y. Que M. Xiong X. Liao N. Xu D. Xu K. Shao B. Zhu (&) Key Laboratory of Ecological Impacts of Hydraulic-Projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources and Chinese Academy of Sciences, Wuhan 430079, People’s Republic of China e-mail: [email protected]; [email protected]
Yangtze river (Jinsha river) and its branch river (Ding 1994). This species has been listed as a near threatened species in IUCN Red List (Zhao 2011). However, due to human activities, such as overfishing, dam constructions, water contamination and habitat alteration, wild fisheries of this species have declined dramatically, the distribution ranges have been shrinking rapidly and even disappear in some river section in the last decades (Li et al. 2013). In the long run, a good understanding of the genetic diversity, population structure and differentiation of P. pingi is required in order to establish adequate management plans for the conservation of this species. Protecting wild P. pingi now has been appraised as critical by our government. However, no suitable nuclear DNA markers are available for P. pingi in the present study. Microsatellites are considered as a powerful genetic marker for population genetic, and are of great utility in species conservation and management. Here we first developed twelve microsatellite loci from P. pingi for the purpose of protecting i
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