Eight novel polymorphic microsatellites in the hispid pocket mouse ( Chaetodipus hispidus ) and cross-amplification in o
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TECHNICAL NOTE
Eight novel polymorphic microsatellites in the hispid pocket mouse (Chaetodipus hispidus) and cross-amplification in other Perognathinae species (Rodentia: Heteromyidae) John J. Andersen • Mark A. Renshaw Jessica E. Light
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Received: 7 June 2012 / Accepted: 23 June 2012 / Published online: 20 July 2012 Ó Springer Science+Business Media B.V. 2012
Abstract Twenty-eight nuclear microsatellites were isolated for the hispid pocket mouse, Chaetodipus hispidus, and characterized in 33 individuals from four genetically distinct groups throughout the geographic range of the species. Dinucleotide and trinucleotide microsatellite repeat motifs were screened, and of the 28 primer pairs created, eight amplified and were polymorphic. After correction for multiple tests, no loci deviated significantly from Hardy– Weinberg expectations. Working primers also were tested in other closely related species found within the subfamily Perognathinae. The microsatellite markers characterized in this study will be beneficial towards future population genetic research within the hispid pocket mouse and other Perognathinae rodents. Keywords Chaetodipus hispidus Heteromyidae Microsatellites Population genetics Rodentia
The hispid pocket mouse, Chaetodipus hispidus (Heteromyidae: Rodentia), occupies a large geographic range extending from North Dakota (Geluso and Wright 2010) to Me´xico. Across this extensive geographic distribution are several barriers to gene flow, such as the Deming Plains (Hunt 1983), Balcones Escarpment (Gehlbach 1991), and Southern Coahuila filter-barrier (Baker 1956). A recent J. J. Andersen M. A. Renshaw J. E. Light (&) Department of Wildlife and Fisheries Sciences, Texas A&M University, College Station, TX 77843, USA e-mail: [email protected] Present Address: M. A. Renshaw Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA
phylogeographic study of C. hispidus found four genetically distinct groups whose geographic limits coincided with these major geographic features (Andersen and Light 2012). Based on these results, Andersen and Light (2012) revised the systematics within the hispid pocket mouse, recognizing the four genetic groups as three subspecies and one incertae sedis. Although this study elucidated the evolutionary history of this widespread species, it used only maternally-inherited markers (i.e., mitochondrial data) and inclusion of nuclear markers (i.e., microsatellites) are necessary to provide a better understanding of the population structure and dynamics within C. hispidus as they relate to historical biogeography. Herein, we characterize eight microsatellite markers for the hispid pocket mouse and determine the utility of these loci across several Perognathinae (heteromyid subfamily which includes the genera Chaetodipus and Perognathus) species. Creation of the enriched genomic library followed the protocol outlined in Welborn et al. (2012). DNA fragments were hybridized with di- and tri-oligonucleotides, incubated with streptavidin-coate
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