Isolation and characterization of fifteen polymorphic tetranucleotide microsatellite loci in Schizopygopsis younghusband
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MICROSATELLITE LETTERS
Isolation and characterization of fifteen polymorphic tetranucleotide microsatellite loci in Schizopygopsis younghusbandi Regan Huan Zheng • Gui-Rong Zhang • Shan-Shan Guo Kai-Jian Wei • Cong-Xin Xie • Tai-Ming Ge
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Received: 30 May 2013 / Accepted: 3 June 2013 Springer Science+Business Media Dordrecht 2013
Abstract Schizopygopsis younghusbandi Regan is an important endemic fish in Tibet, China. Its wild population has been rapidly declining for the past few decades due to overfishing, dam construction and invasion of exotic fishes. To conserve natural resources and develop breeding stocks of S. younghusbandi, fifteen polymorphic tetranucleotide microsatellite loci were isolated and characterized in 45 individuals collected from the Yarlung Tsangpo River in Tibet. The number of alleles per locus ranged from 6 to 32 with an average of 17.87. The expected heterozygosity and Shannon–Wiener diversity index ranged from 0.454 to 0.951 and from 0.931 to 3.201, respectively. These microsatellite loci will be useful for future population genetic analysis and resource conservation of S. younghusbandi. Keywords Schizopygopsis younghusbandi Microsatellites Genetic diversity Polymorphism
H. Zheng G.-R. Zhang S.-S. Guo K.-J. Wei (&) C.-X. Xie Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, People’s Republic of China e-mail: [email protected] H. Zheng G.-R. Zhang S.-S. Guo K.-J. Wei Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, Wuhan 430070, People’s Republic of China T.-M. Ge (&) Hubei Key Laboratory of Wetland Evolution and Ecological Restoration, China University of Geosciences, Wuhan 430074, People’s Republic of China e-mail: [email protected]
Schizopygopsis younghusbandi Regan (Cyprinidae: Schizothoracinae) is an endemic tetraploid species that is mainly distributed in the middle reaches of the Yarlung Tsangpo River in Tibet, where it is one of the most important commercial fish species (Fisheries Bureau of Tibet Autonomous Region 1995; Wu et al. 1999). It is a cold-adapted cyprinid fish that grows slowly and matures late as adaptation to its rigorous environment. For the past few decades, S. younghusbandi has experienced a rapid decline in wild population size due to overfishing, dam construction and invasion of exotic fishes. To conserve natural resources and develop breeding stocks of S. younghusbandi, it is especially necessary to study its genetic diversity and population genetic structure. Microsatellites are highly informative genetic markers and widely used in population genetics studies. However, there is little information about population genetic status of this species except for limited studies on its chromosome, phylogeny and some biological characteristics. In this study, fifteen polymorphic tetranucleotide microsatellite loci were isolated and characterized in S. younghusbandi in order to further study its genetic diversity and population ge
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