Development of consolidated bioprocess for biofuel-ethanol production from ultrasound-assisted deep eutectic solvent pre
- PDF / 1,394,547 Bytes
- 16 Pages / 595.276 x 790.866 pts Page_size
- 56 Downloads / 188 Views
ORIGINAL ARTICLE
Development of consolidated bioprocess for biofuel-ethanol production from ultrasound-assisted deep eutectic solvent pretreated Parthenium hysterophorus biomass Parushi Nargotra 1 & Vishal Sharma 1 & Surbhi Sharma 1 & Nisha Kapoor 1 & Bijender Kumar Bajaj 1 Received: 2 July 2020 / Revised: 1 September 2020 / Accepted: 12 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A novel consolidated bioprocess was designed for processing of Parthenium hysterophorus biomass based on ultrasoundassisted deep eutectic solvent (DES) pretreatment followed by in situ enzymatic saccharification and fermentation of sugars to ethanol-biofuel. Among various DESs examined, choline chloride/sorbitol (CCS, molar ratio 1:5) pretreated P. hysterophorus biomass yielded maximum sugar (148.54 mg/g biomass) upon enzymatic saccharification. Furthermore, combined ultrasonic irradiation and CCS pretreatment resulted in substantially enhanced sugar yield as compared with either of the standalone pretreatment. A central composite design was employed for the optimization of combined ultrasound and choline chloride/ sorbitol pretreatment that further aided in achieving increased reducing sugar yield (25.72%). The pretreated biomass was examined by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and proton nuclear magnetic resonance (1H-NMR) analysis to elucidate the functional mechanisms of combinative pretreatment. Understanding of pretreatment mechanisms may help custom designing of novel strategies for the efficient refining of biomass. Keywords Deep eutectic solvents . Ultrasound . Consolidated bioprocessing . P. hysterophorus . Pretreatment . Bioethanol
1 Introduction Lignocellulosic bioethanol (second-generation biofuel), owing to its desirable properties, may have the potential to emerge as the most suitable and renewable fuel alternative to circumvent the issues like rapidly dwindling conventional fossil fuels and their environmental implications [1, 2]. The bioethanol generated from lignocellulosic feedstocks Highlights • First report on bioprocessing of P. hysterophorus using deep eutectic solvents, DESs. • Ultrasound-assisted DES pretreatment enhances the sugar yield and hence the process efficacy. • A one-pot consolidated process was developed for combined DES and ultrasound pretreatment. • Molecular mechanism of pretreatment was studied by SEM, FT-IR, XRD and 1H-NMR • Consolidated one-pot bioprocess for the conversion of biomass to biofuel-ethanol. * Bijender Kumar Bajaj [email protected] 1
School of Biotechnology, University of Jammu, Jammu, India
(agricultural/forestry residues, weeds, municipal waste, etc.) may potentially feed the industrial and transport sectors to minimize the dependence on fossil fuels and to sustain a healthy ecosystem [3–5]. However, most of the research for bioethanol production has been based on single feedstock involving agricultural crop waste, whose supply throughout the year is unwar
Data Loading...
