Development of microsatellite markers for the predatory mite Phytoseiulus macropilis and cross-amplification in three ot
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Development of microsatellite markers for the predatory mite Phytoseiulus macropilis and cross‑amplification in three other species of phytoseiid mites Maria Cristina Vitelli Queiroz1 · Fernanda Ancelmo de Oliveira2 · Anete Pereira de Souza2 · Mario Eidi Sato1 Received: 27 May 2020 / Accepted: 9 November 2020 © Springer Nature Switzerland AG 2020
Abstract Phytoseiid mites are efficient predators of mites and small pest insects. Understanding the dispersion and distribution pattern of phytoseiid mites is essential to promote the conservation of these natural enemies and support their use in biological control. Population genetic studies using molecular markers such as microsatellites have proved to be extremely informative to address questions about population structure and dispersion patterns of predatory mites. The objective of this work was to develop specific microsatellite markers for the predatory mite Phytoseiulus macropilis, aiming at improving field dispersion studies. For this purpose, the genomic DNA was extracted from the whole body of a pool of 260 adult females and used to build the genomic microsatellites-enriched library, using biotinylated probes (CT)8 and (GT)8. In total 26 pairs of primers were synthesized and screened across 30 adult females of P. macropilis for characterization. Seven loci were polymorphic, revealing from two to six alleles per locus. Cross amplifications were successfully obtained in the species Phytoseiulus persimilis, Amblyseius swirskii and Proprioseiopsis sp. The molecular markers obtained are the first developed for P. macropilis—they are effective for the detection and quantification of genetic variation, and show high transferability, thus can be used in genetic and molecular studies of this and other species of the same genus and also of close genera. Keywords Pyrethroid resistance · SSR · Molecular markers · Phytoseiulus persimilis · Amblyseius swirskii
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s1049 3-020-00572-5) contains supplementary material, which is available to authorized users. * Maria Cristina Vitelli Queiroz [email protected] 1
Laboratório de Acarologia, Instituto Biológico, APTA, Rodovia Heitor Penteado km 3.5, Caixa Postal 70, Campinas, SP 13001‑970, Brazil
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Plant Biology Department, Biology Institute, University of Campinas (UNICAMP), Cidade Universitária Zeferino Vaz, CP 6109, Campinas, SP CEP 13083‑970, Brazil
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Experimental and Applied Acarology
Introduction Phytoseiid mites are efficient predators of mites and small insects (Gerson et al. 2003). Some species are commercialized for biological control of agricultural pests. Among these species, two belong to the genus Phytoseiulus: Phytoseiulus macropilis (Banks) and Phytoseiulus persimilis Athias-Henriot. Both species are specialized predators (McMurtry et al. 2013), preying mainly on Tetranychus urticae Koch (Acari: Tetranychidae), which is a polyphagous and cosmopolitan species, feeding and causing
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