Development of nanoemulsion formulation of mustard oil, its chemical characterization and evaluation against post harves
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RESEARCH ARTICLE
Development of nanoemulsion formulation of mustard oil, its chemical characterization and evaluation against post harvest anthracnose pathogens Aloke Purkait1 · Rodas Ezezew Worede2 · Deewakar Baral3 · Dipak Kumar Hazra2 · Birendra Nath Panja3 · Pabitra Kumar Biswas1 · Ramen Kumar Kole2 Received: 9 February 2020 / Revised: 6 April 2020 / Accepted: 1 May 2020 © Indian Phytopathological Society 2020
Abstract Oil-in-water nanoemulsion (NE) formulations of mustard oil known for prominent antimicrobial activities have been prepared, characterized and tested against anthracnose pathogens Colletotrichum musae and Colletotrichum capsici. Physicochemical properties viz., appearance, stability, pH, centrifugation, storage-stability, thermodynamic stress, persistent foam, droplets size and zeta potential were tested for the NE formulations. The NE formulations were prepared using low energy emulsification process with the optimized composition (w/w) of mustard oil (5%), Tween 20 and sodium dodecyl benzene sulfonate emulsifiers blend (20–30%), co-surfactants (4%), and de-ionized water. The required hydrophile-lipophile balance value for emulsifier blends was determined as 11.39–11.74. Only 3 (viz., NF2, NF3 and NF6) out of 15 NE formulations with transparent faint yellow color (pH 6.3) passed all the physico-chemical parameters tested. Among these, NF2 (foam height
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