Dietary fatty acids and the time elapsed from their intake are related to their composition in rat submandibular gland a
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ORIGINAL ARTICLE
Dietary fatty acids and the time elapsed from their intake are related to their composition in rat submandibular gland and salivary flow rates Jorge Escandriolo Nackauzi 1,2 & Gastón Repossi 3 & Claudio Bernal 4 & Adriana Actis 3 & Raquel Gallará 2 Received: 19 August 2019 / Accepted: 14 April 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Objectives The aim of this study was to analyze the influence of dietary fatty acids (FAs) and the time elapsed from their intake on FA tissue profile of rat submandibular gland (SG) and on its salivary flow rate (SFR). Do dietary FAs depending on the intake time modify their profile in SG and consequently the SFR? Materials and methods Thirty-six adult male Wistar rats were fed on control diet (corn oil, CD, 18:2 n-6 FA) for 7 days and then divided into CD and two groups with replacement of corn oil by olive (OD, 18:1 n-9 FA) or chia (ChD, 18:3 n-3 FA) oils (1 and 30 day intake). Submandibular ducts were canalized to collect saliva for 20 min (μL/min). SG were examined (optical/electron microscopy; ImageJ 1.48 software). Results SFR values were 6.18 ± 0.34 (CD1), 6.04 ± 0.31 (OD1), and 6.00 ± 0.50 (ChD1) (p > 0.05). At 30-day intake, higher SFR values in ChD (7.82 ± 0.7) with respect to CD (4.68 ± 0.44; p < 0.001) and OD (6.08 ± 0.2; p = 0.038) were found. ChD30 showed a higher serous acinous area percentage than CD30 and OD30, whereas mucous acinous density was greater in CD30 than in OD30 and ChD30 (p < 0.05). α-Linolenic (ALA) and eicosapentaenoic and docosahexaenoic acid levels were only detected in SG of ChD30, while arachidonic acid was lower in this group as compared with CD30 and OD30 (p < 0.05). Conclusions SG FA composition and its SFR appear to be modulated by dietary FAs and the time elapsed from their consumption. SFR is highest with n-3 ALA-rich ChD at 30-day intake. Clinical relevance Diet could contribute to improve secretory dysfunctions. Keywords Salivary flow rates . Fatty acids . Dietary intake . Submandibular gland
Introduction Salivary glands are involved in secretion of saliva which is a biological fluid that promotes tooth remineralization, protects against infections, allows taste perception of foods, and initiates digestion, among other functions [1–3]. A profuse secretion is
mainly induced during the eating process by physiological stimulation (stimulated saliva). In addition, a lesser amount of unstimulated saliva which reflects basal salivary flow rate (SFR) is also produced. The whole unstimulated saliva— mostly secreted by the submandibular gland—is the most important in the maintenance of the oral and gastrointestinal
Actis AB and Gallará RV are co-last authors. * Jorge Escandriolo Nackauzi [email protected]; [email protected] Gastón Repossi [email protected] Claudio Bernal [email protected] Adriana Actis [email protected] Raquel Gallará [email protected]
1
Facultad de Ciencias de la Salud, Universidad Adventista de Plata, 25 de mayo 99, 3103 Liber
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