Distinct serum metabolomic signatures of multiparous and primiparous dairy cows switched from a moderate to high-grain d
- PDF / 5,671,406 Bytes
- 18 Pages / 595.276 x 790.866 pts Page_size
- 17 Downloads / 158 Views
ORIGINAL ARTICLE
Distinct serum metabolomic signatures of multiparous and primiparous dairy cows switched from a moderate to high‑grain diet during early lactation C. Pacífico1 · A. Stauder1 · N. Reisinger3 · H. E. Schwartz‑Zimmermann2 · Q. Zebeli1 Received: 29 April 2020 / Accepted: 18 August 2020 © The Author(s) 2020
Abstract Introduction Feeding of high-grain diets is common in cows during early lactation, but increases the odds of metabolic derailments, which can likely be detected as undesirable shifts in the serum metabolome signature. Objectives The present study aimed to identify the metabolic signatures of the serum metabolome of early lactation dairy cows switched from a moderate to a high-grain diet. Methods Targeted ESI-LC-MS/MS-based metabolomics was used to characterize metabolic alterations in the serum of early lactation multiparous (MP, n = 16) and primiparous (PP, n = 8) Simmental cows, according to parity and feeding phase. Data were analysed using different data mining approaches. Results Carnitine, acetylcarnitine, propionoylcarnitine, amino acid related compounds cis-4-hydroxyproline, trans-4-hydroxyproline, proline betaine, lysophosphatidylcholine PC a C16:1 and phosphatidylcholine PC ae C36:0 were identified as the key metabolites distinguishing MP from PP cows. A different serum metabolite composition during moderate and high-grain diet was also evident. Notably, cows fed high grain diet had higher serum concentrations of primary bile acids and triglycerides, but lower levels of conjugated bile acids and carboxylic acids during the first week in grain. Amino acids valine, cystine and taurine together with lysophosphatidylcholine PC a C26:0 and several phosphatidylcholines were classified as important features for cluster separation. Conclusions Our study greatly expands earlier observations on dietary effects on serum metabolome composition of cows. The altered metabolomic fingerprints clearly distinguishable by diet and cow parity hold potential to be used as early diagnostic tools for cows experiencing grain-induced metabolic disturbances. Keywords Ruminal acidosis · Parity · Dairy cow · Early lactation · Targeted metabolomics Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11306-020-01712-z) contains supplementary material, which is available to authorized users. * Q. Zebeli [email protected] 1
Christian Doppler Laboratory for Innovative Gut Health Concepts of Livestock, Institute of Animal Nutrition and Functional Plant Compounds, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria
2
Christian Doppler Laboratory for Innovative Gut Health Concepts of Livestock, Institute of Bioanalytics and Agro‑Metabolomics, Department of Agrobiotechnology (IFA‑Tulln), University of Natural Resources and Life Sciences, Vienna, Austria
3
BIOMIN Research Center, BIOMIN Holding GmbH, Tulln, Austria
1 Introduction In dairy cows, early lactation represents both
Data Loading...
