Time-dependent mRNA expression of selected pro-inflammatory factors in the endometrium of primiparous cows postpartum
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RESEARCH
Open Access
Time-dependent mRNA expression of selected pro-inflammatory factors in the endometrium of primiparous cows postpartum Christoph Gabler1*, Claudia Fischer1, Marc Drillich2,3, Ralf Einspanier1, Wolfgang Heuwieser2
Abstract Background: Inflammatory processes and infections of the uterine wall must be accepted as a physiological event in dairy cows after calving. This might result in clinical or subclinical endometritis which is assumed to impair reproductive performance in the current lactation. Several cytokines and acute phase proteins have been discussed as local and systemic mediators of these inflammatory processes. The aim of the present study was to investigate the endometrial mRNA expression of the chemokine CXC ligand 5 (CXCL5), interleukin 1b (IL1B), IL6, IL8, tumour necrosis factor alpha (TNF), prostaglandin-endoperoxide synthase 2 (PTGS2) and haptoglobin (HP) in the postpartum period. Methods: Endometrial samples were obtained from primiparous cows (n = 5) on days 10, 17, 24, 31, 38 and 45 postpartum (pp) using the cytobrush technique. Cytological smears were prepared from cytobrush samples to determine the proportion of polymorphonuclear neutrophils (PMN). Total RNA was extracted from endometrial samples, and real-time RT-PCR was performed. Results: A time-dependent mRNA expression of the investigated factors was found for the course of the postpartum period. In detail, a significantly higher expression of these factors was observed on day 17 pp compared to day 31 pp. Furthermore, the proportion of PMN peaked between days 10-24 pp and decreased thereafter to low percentages (< 5%) on day 31 pp and thereafter. In addition, CXCL5, IL1B, IL8 and HP mRNA expression correlated significantly with the proportion of PMN (P < 0.05). A significantly higher CXCL5, IL1B, IL6, IL8, PTGS2 and TNF mRNA content was observed in samples from cows with an inflamed endometrium compared with samples from cows with a healthy endometrium (P < 0.05). Conclusions: These results show that inflammatory cytokines and acute phase proteins are expressed in the bovine endometrium in a time-related manner during the postpartum period, with a significant expression peak on day 17 pp as a possible mucosal immune response in the uterus. The evaluation of the expression patterns of such candidate genes may reveal more information than only determining the percentage of PMN to judge the severity of an inflammation.
Background Invasion of aerobic and anaerobic bacteria into the uterus is common in dairy cattle after parturition. The uterine bacterial colonisation is non-specific and includes a variety of pathogenic bacteria, e.g. Escherichia coli, Arcanobacterium pyogenes, Fusobacterium necrophorum and Prevotella species. Within 2-4 weeks after parturition most of the bacteria are eliminated spontaneously [1]. * Correspondence: [email protected] 1 Institute of Veterinary Biochemistry, Freie Universität Berlin, Oertzenweg 19b, 14163 Berlin, Germany Full list of author information is available at the end of th
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