Mitochondrial DNA Methods and Protocols
Since the publication of the first edition, the number of unique heritable mtDNA mutations recognized as being associated with bioenergetic dysfunction, cell death and disease has grown. Likewise, our understanding of the basic biology of somatic mtDNA mu
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IN
M O L E C U L A R B I O L O G Y TM
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For other titles published in this series, go to www.springer.com/series/7651
Mitochondrial DNA Methods and Protocols
Edited by
Jeffrey A. Stuart Department of Biological Sciences, Brock University, St. Catharines, ON, Canada
Editor Jeffrey A. Stuart Department of Biological Sciences Brock University 500 Glenridge Avenue Cpy Catharines ON L2S 3A1 Canada [email protected] Series Editor John M. Walker University of Hertfordshire Hatfield, Herts UK
ISSN 1064-3745
e-ISSN 1940-6029
ISBN 978-1-934115-60-2 e-ISBN 978-1-59745-521-3 DOI 10.1007/978-1-59745-521-3 Springer Dordrecht Heidelberg London New York Library of Congress Control Number: 2009926583 # Humana Press, a part of Springer ScienceþBusiness Media, LLC 2009 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer ScienceþBusiness Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper 98765432
Corrected at second printing 2009
Springer is part of Springer ScienceþBusiness Media (www.springer.com)
Preface Since the publication of the first edition of Mitochondrial DNA: Methods and Protocols in 2002, the number of unique heritable mtDNA mutations recognized as being associated with bioenergetic dysfunction, cell death, and disease has grown apace. At the same time, our understanding of the basic biology of somatic mtDNA mutations has improved. These ongoing advancements are due largely to the continuous development and improvement of techniques and approaches for studying the biology of mitochondria and their DNA. In this second edition of Mitochondrial DNA: Methods and Protocols, specialists from eight countries share their expertise by providing detailed protocols for studying many aspects of mtDNA. This volume is divided into three sections. The first contains protocols that can be used to study the transduction of information from mtDNA to functionally active respiratory complexes. Included in this section are protocols for investigating the nucleoid proteome, mtDNA packaging, replication, transcription, and respiratory complex synthesis. In this section, methods for studying polymerase gamma mutations associated with mitochondrial disorders are also provided. The second section focuses on mitochondrial reactive oxygen species (ROS) production, mtDNA damage,
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