DNA-Protein Interactions Principles and Protocols
This fourth edition reflects the most recent technical advances in DNA-protein interaction research. With fully updated chapters that describe techniques proven by their continuous value, the volume also many new chapters have been added that mostly deal
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Benoît P. Leblanc Sébastien Rodrigue Editors
DNA-Protein Interactions Principles and Protocols Fourth Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
DNA-Protein Interactions Principles and Protocols Fourth Edition
Edited by
Benoît P. Leblanc and Sébastien Rodrigue Département de Biologie, Faculté des Sciences, Université de Sherbrooke, Sherbrooke, QC, Canada
Editors Benoît P. Leblanc Département de Biologie, Faculté des Sciences Université de Sherbrooke Sherbrooke, QC, Canada
Sébastien Rodrigue Département de Biologie, Faculté des Sciences Université de Sherbrooke Sherbrooke, QC, Canada
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2876-7 ISBN 978-1-4939-2877-4 (eBook) DOI 10.1007/978-1-4939-2877-4 Library of Congress Control Number: 2015945654 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface Science and technology have gone hand in hand for several centuries now, to the point where they are essentially synonymous in the mind of the general public. Considering the immense progress made since the Enlightenment in the fields of chemistry, physics, and biology (to name only those disciplines) due to the availability of ever more sophisticated measuring devices, we must admit, if a bit ruefully, that our understanding of the universe would have been a very limited thing indeed if all we had to rely on had been our five senses and our intelligence. And so a curious and industrious humanity built tools that would extend the reach of its limited senses to bring previously unreachable
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