Donepezil attenuates injury following ischaemic stroke by stimulation of neurogenesis, angiogenesis, and inhibition of i
- PDF / 4,415,691 Bytes
- 14 Pages / 595.276 x 790.866 pts Page_size
- 30 Downloads / 176 Views
Inflammopharmacology
ORIGINAL ARTICLE
Donepezil attenuates injury following ischaemic stroke by stimulation of neurogenesis, angiogenesis, and inhibition of inflammation and apoptosis Arian Madani Neishaboori1,2 · Solmaz Nasseri Maleki1,2 · Mahdi Saberi Pirouz1,2 · Sara Golmohammadi1,2 · Donya Nazarinia3 · Nahid Aboutaleb1,2 Received: 13 January 2020 / Accepted: 5 October 2020 © Springer Nature Switzerland AG 2020
Abstract Donepezil has proven to be an effective drug to reduce neuronal death and subsequently injury in neurodegenerative diseases. The current study evaluated the neuroprotective effects of donepezil in a rat model of ischaemic stroke and explored possible mechanisms which by this drug may reduce cell death. Temporary middle cerebral artery occlusion (tMCAO) was exerted for 45 min to induce ischaemic stroke. The animals were assigned into five groups: sham, control, and three groups treated with different doses of donepezil. Donepezil was intraperitoneally (IP) injected 4 h after reperfusion for 10 consecutive days. Infarct size was determined using TTC staining. The expression of proteins was evaluated using immunohistochemistry assays. Compared with the control group, infarct size was significantly reduced in tMCAO rats treated with different doses of donepezil. Moreover, our results showed significant decreased expression levels of apoptotic markers and pro-inflammatory mediators after treatment with different doses of donepezil for 10 days (P
Data Loading...
