Early nodulin 93 protein gene: essential for induction of somatic embryogenesis in oil palm
- PDF / 2,392,104 Bytes
- 19 Pages / 595.276 x 790.866 pts Page_size
- 34 Downloads / 133 Views
ORIGINAL ARTICLE
Early nodulin 93 protein gene: essential for induction of somatic embryogenesis in oil palm Pek‑Lan Chan1,3 · Ray J. Rose2 · Abdul Munir Abdul Murad3 · Zamri Zainal3 · Pei‑Wen Ong1,5 · Leslie Cheng‑Li Ooi1 · Eng‑Ti Leslie Low1 · Zamzuri Ishak1,6 · Suzaini Yahya4,7 · Youhong Song2,8 · Rajinder Singh1 Received: 10 March 2020 / Accepted: 16 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message Transcript profiling during the early induction phase of oil palm tissue culture and RNAi studies in a model somatic embryogenesis system showed that EgENOD93 expression is essential for somatic embryogenesis. Abstract Micropropagation of oil palm through tissue culture is vital for the generation of superior and uniform elite planting materials. Studies were carried out to identify genes to distinguish between leaf explants with the potential to develop into embryogenic or non-embryogenic callus. Oil palm cDNA microarrays were co-hybridized with cDNA probes of reference tissue, separately with embryo forming (media T527) and non-embryo (media T694) forming leaf explants sampled at Day 7, Day 14 and Day 21. Analysis of the normalized datasets has identified 77, 115 and 127 significantly differentially expressed genes at Day 7, Day 14, and Day 21, respectively. An early nodulin 93 protein gene (ENOD93), was highly expressed at Day 7, Day 14, and Day 21 and in callus (media T527), as assessed by RT-qPCR. Validation of EgENOD93 across tissue culture lines of different genetic background and media composition showed the potential of this gene as an embryogenic marker. In situ RNA hybridization and functional characterization in Medicago truncatula provided additional evidence that ENOD93 is essential for somatic embryogenesis. This study supports the suitability of EgENOD93 as a marker to predict the potential of leaf explants to produce embryogenic callus. Crosstalk among stresses, auxin, and Nod-factor like signalling molecules likely induces the expression of EgENOD93 for embryogenic callus formation. Keywords Early nodulin 93 protein gene · Elaeis guineensis · Somatic embryogenesis · Nodulation · Microarray · Medicago truncatula
Introduction
Communicated by Neal Stewart. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00299-020-02571-7) contains supplementary material, which is available to authorized users. * Pek‑Lan Chan [email protected] 1
2
3
Advanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board (MPOB), No. 6, Persiaran Institusi, Bandar Baru Bangi, 43000 Kajang, Selangor, Malaysia School of Environmental and Life Sciences, The University of Newcastle, Callaghan, NSW 2308, Australia School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia
The indirect somatic embryogenesis (SE) process in oil palm is initiated from leaf explants, which dedifferentiate into callus followed by somatic embryo formatio
Data Loading...
