Induction of somatic embryogenesis in different explants from Ormosia henryi Prain
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ORIGINAL ARTICLE
Induction of somatic embryogenesis in different explants from Ormosia henryi Prain Gao‑Yin Wu1,2 · Xiao‑Li Wei1,2 · Xiao Wang1,2 · Yi Wei1,2 Received: 6 January 2020 / Accepted: 4 April 2020 © Springer Nature B.V. 2020
Abstract An efficient in vitro plant regeneration method via somatic embryogenesis has been established in Ormosia henryi Prain, which is an important precious tree in the timber industry. Five types of explants, six types of basic media and different plant growth regulators (PGRs) were used for embryogenic callus (EC) initiation, EC proliferation, somatic embryo (SE) induction and SE germination. The EC and SE induction rates from mature zygotic embryos (MZE) were higher than those from stem segments, cotyledons, leaves and hypocotyls. MZE were used as explants, and the EC induction rate was greater than 30% on B5 medium supplemented with 1.0 mg/l 6-benzylaminopurine (BA) and 0.5 mg/l naphthaleneacetic acid (NAA) or 0.2 mg/l BA and 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). The highest induction rate (22.83%) for SE was obtained with B5 medium supplemented with 0.5 mg/l kinetin (KT) and 1.0 mg/l 2,4-D. The EC proliferation rate and SE number in the suspension culture were increased compared to those in the solid culture. Fifty-four percent of SE developed to the cotyledonary stage on B5 medium supplemented with 0.5 mg/l BA and 0.2 mg/l NAA. The number of cotyledon embryos was the highest on medium supplemented with 0.5 mg/l thidiazuron (TDZ) and 0.2 mg/l NAA. Histological analysis confirmed the bipolar organization of the somatic embryos, which were comprised of shoot and root meristems, and showed that SE was characterized by globular, heart-shaped, torpedo-shaped and cotyledon stages in O. henryi, the developmental process of which was similar to that of zygotic embryos. Key Message An efficient in vitro plant regeneration method via somatic embryogenesis has been established in Ormosia henryi Prain using mature zygotic embryos as explant. Keywords Ormosia henryi · Embryogenic callus · Somatic embryogenesis · Mature embryos · Suspension culture
Introduction Ormosia henryi Prain is a precious tree species in China and has great economic and ecological potential as a raw material for making high-grade furniture and handicrafts because its wood is dense and hard and its wood texture is Communicated by Maria Margarida Oliveira. * Xiao‑Li Wei gdwxl‑[email protected] 1
College of Forestry, Guizhou University, Guiyang 550025, Guizhou, China
Institute for Forest Resources and the Environment of Guizhou, Guizhou University, Guiyang 550025, Guizhou, China
2
beautiful; in addition, it functions as a superior fireproof tree species in the forest. There are some factors that restrict the propagation and breeding of O. henryi, such as a lack of wild resources, seed pests, dormancy characteristics due to hard seeds, animal feeding and deleterious alternate bearing phenomenon (Xiaoli et al. 2014). Therefore, it is very important to find a fast and efficient method of reproduction fo
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