Ectopic expression of nucleolar DEAD-Box RNA helicase OsTOGR1 confers improved heat stress tolerance in transgenic Chine
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ORIGINAL ARTICLE
Ectopic expression of nucleolar DEAD‑Box RNA helicase OsTOGR1 confers improved heat stress tolerance in transgenic Chinese cabbage Rajesh Yarra1 · Yongbiao Xue1,2 Received: 17 August 2020 / Revised: 13 September 2020 / Accepted: 18 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Key message The DEAD-Box RNA helicase OsTOGR1 positively regulates heat stress tolerance in Chinese cabbage. Abstract Non-heading Chinese cabbage (Brassica rapa L. ssp. chinensis) is primarily cultivated vegetable crop in Asian countries. Heat stress is one of the major threats for its growth and yield. Numerous regulatory genes in various crops have shown to contribute thermotolerance. Among them, Thermotolerant growth required 1 (TOGR1) is an important DEAD-box RNA helicase. To examine whether its role is conserved in other crops, we constructed pCAMBIA1300-pHSP:OsTOGR1 expression vector driven by the rice small heat shock protein promoter (pHSP17.9) and successfully produced transgenic non-heading Chinese cabbage plants expressing OsTOGR1 gene via Agrobacterium-mediated vacuum infiltration transformation. In total, we generated three independent transgenic cabbage lines expressing TOGR1 gene. Expression and integration of TOGR1 was confirmed by PCR, RT-PCR and qPCR in T 1 and T 2 generations. The relative leaf electrical conductivity of transgenic seedlings was reduced subjected to high temperature (38 °C) compared to heat shock treatment (46 °C). In addition, hypocotyl length of transgenic seedlings increased compared to wild-type plants under high temperature and heat shock treatment. Furthermore, the transgenic plants exhibited higher chlorophyll content than wild-type plants under high temperature and heat shock treatment. The transgenic seeds displayed better germination under heat shock treatment. Tested heat stress-responsive genes were also up-regulated in the transgenic plants subjected to high temperature or heat shock treatment. To the best of our knowledge, this is the first report on describing the role of DAED-Box RNA helicases in improving heat stress tolerance of transgenic plants. Keywords TOGR1 · thermotolerant growth required 1 · HSP · heat shock protein · Non-heading Chinese cabbage
Introduction The sixth report of the Intergovernmental Panel on Climate Change mission aspires to abate the rise in earth’s temperature (Xu et al. 2018). High temperature is considered to be a major environmental factor limiting crop growth and productivity (Zhang et al. 2019; Driedonks et. al. 2016; Communicated by Günther Hahne. * Yongbiao Xue [email protected] 1
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
University of Chinese Academy of Sciences, Beijing 100049, China
2
Gangadhar et al. 2016). Heat stress, 5 °C above the optimal growth temperature of plants induces an array of cellular and molecular changes to withstand high-temperature cond
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