Ectopic expression of MYB repressor GmMYB3a improves drought tolerance and productivity of transgenic peanuts ( Arachis
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ORIGINAL PAPER
Ectopic expression of MYB repressor GmMYB3a improves drought tolerance and productivity of transgenic peanuts (Arachis hypogaea L.) under conditions of water deficit Yuxuan He . Shujing Mu . Zhongguo He . Baizhong Wang . Yufa Li
Received: 16 February 2020 / Accepted: 24 October 2020 Ó Springer Nature Switzerland AG 2020
Abstract Peanut is widely grown and provides protein and edible oil for millions of people. Peanut growth and productivity are frequently negatively affected by abiotic and biotic environmental factors. However, the research on improving peanut germplasm resources by genetic transformation is very limited. Here, the novel R2R3-MYB repressor GmMYB3a was introduced into peanut plants by Agrobacterium-mediated transformation for the first time for thorough evaluation of the function of GmMYB3a in drought stress plant responses. We generated GmMYB3a-transgenic peanut plants. The GmMYB3a-overexpressing lines showed significantly improved physiological responses and no yield loss non-transgenic plants, in terms of survival rates. Thus, the GmMYB3a-overexpressing plants showed better photosynthetic performance, higher relative water content, and greater water use efficiency, demonstrating their adaptive capacity to water deficit. We conclude that overexpression of GmMYB3a can improve drought tolerance and productivity in peanut.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11248-020-00220-z) contains supplementary material, which is available to authorized users. Y. He S. Mu Z. He B. Wang Y. Li (&) Jilin Academy of Agricultural Sciences, Changchun 130033, China e-mail: [email protected]
Keywords Arachis hypogaea L. Drought stress Ectopic expression MYB Transgenic peanut lines
Introduction Peanut or groundnut (Arachis hypogaea L.) is rich in a variety of edible oil, vitamins, and nutritive proteins. Peanut is widely grown in semiarid tropical regions of Asia, Africa, North, and South America (Mace et al. 2006; Cuc et al. 2008). Drought due to erratic rainfall may vary in intensity and duration, as well as increased temperature associated with water deficit, resulting in varying degrees of plant stress. The problem is difficult to solve by conventional approaches. Globally, peanut yield losses caused by drought stress come close to 6 million tons (Bhatnagar-Mathur et al. 2007). Screening peanut germplasm for stress resistance has had little success due to scarce genotypic variability and to the rarity of alleles (Bhatnagar-Mathur et al. 2007). In addition to traditional breeding, molecular breeding is considered as an important and effective direction for research in plant tolerance to stress (Xiong and Zhu 2002). The introgression caused undesirable gene blocks transferred from wild to cultivated peanuts can be overcome through the identification of suitable genes by genetic transformation (Kumar and Kirti 2015). Marker-assisted selection (MAS) is a significant
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