Effect of a 2000-m running test on antioxidant and cytokine response in plasma and circulating cells
- PDF / 406,250 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 69 Downloads / 155 Views
ORIGINAL ARTICLE
Effect of a 2000-m running test on antioxidant and cytokine response in plasma and circulating cells Lucrecia Carrera-Quintanar 1 & Lorena Funes 2 & Miguel Sánchez-Martos 2 & Pascual Martinez-Peinado 3 & José M. Sempere 3 & Antoni Pons 4,5 & Vicente Micol 2,5 & Enrique Roche 1,5,6
Received: 9 May 2016 / Accepted: 29 June 2017 # University of Navarra 2017
Abstract Exercise intensity usually correlates with increased oxidative stress and enhanced cytokine production. However, it is unknown if all types of exercise that induce muscle damage can cause a parallel response in the oxidation balance and cytokine production. To this end, the effect of a 2000-m running test in a group of volunteers that regularly train in aerobic routines was studied. Different circulating parameters were measured, oxidative stress markers (protein carbonyls and malondialdehyde), antioxidant enzyme activity, and cytokine levels in plasma as well as in the main circulating cells of blood samples obtained in basal conditions and after test execution. As a result, the test caused muscle damage evidenced by an increase in circulating creatine kinase and myoglobin. This was accompanied by an increase in protein carbonyls in plasma and peripheral blood mononuclear cells. Activities of antioxidant enzymes (catalase, glutathione peroxidase and reductase, superoxide dismutase) were elevated in peripheral * Enrique Roche [email protected] 1
Department of Applied Biology-Nutrition, University Miguel Hernandez, Alicante Institute for Health and Biomedical Research (ISABIAL-FISABIO Foundation), Alicante, Spain
2
Institute for Molecular and Cellular Biology, University Miguel Hernandez, Elche (Alicante), Spain
3
Immunology Division, Biotechnology Department, University of Alicante, Alicante, Spain
4
Department for Basic Biology and Health Sciences, University of Balearic Islands, Palma de Mallorca, Spain
5
CIBERobn (Fisiopatología de la Obesidad y la Nutrición CB12/03/ 30038) Instituto de Salud Carlos III, Madrid, Spain
6
Departamento de Biología Aplicada e Instituto de Bioingeniería, Área de Nutrición y Bromatología, Universidad Miguel Hernández, Avda de la Universidad sn., 03202 Elche (Alicante), Spain
blood mononuclear cells, neutrophils, and erythrocytes after the test. Regarding cytokine production, interleukin-6, interleukin-8, interleukin-10, and tumor necrosis factor-α exhibited no significant changes after the test. Results suggest that this short but intense running exercise (2000 m) can induce muscle damage and elicit a good balance between oxidant/ antioxidant responses with no changes in the circulating concentration of pro-inflammatory cytokines. Keywords Antioxidants . Inflammation . Interleukins . Oxidative stress
Introduction Intense physical activity induces a series of alterations in many body compartments, which is reflected in changes in circulating parameters. Plasma alterations include an increased presence of tissue proteins as a consequence of the stress inherent to exercise. The majority of these circ
Data Loading...
