Effect of different muscle contraction mode on the expression of Myostatin, IGF-1, and PGC-1 alpha family members in hum
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ORIGINAL ARTICLE
Effect of different muscle contraction mode on the expression of Myostatin, IGF-1, and PGC-1 alpha family members in human Vastus Lateralis muscle Pejman Taghibeikzadehbadr1 · Sadegh Shirian2,3,4 · Mostafa Sabouri1 Received: 17 June 2020 / Accepted: 16 November 2020 © Springer Nature B.V. 2020
Abstract Muscle contraction stimulates a transient change of myogenic factors, partly related to the mode of contractions. Here, we assessed the response of IGF-1Ea, IGF-1Eb, IGF-1Ec, PGC1α-1, PGC1α-4, and myostatin to the eccentric Vs. the concentric contraction in human skeletal muscle. Ten healthy males were performed an acute eccentric and concentric exercise bout (n = 5 per group). For each contraction type, participants performed 12 sets of 10 repetitions knee extension by the dominant leg. Baseline and post-exercise muscle biopsy were taken 4 weeks before and immediately after experimental sessions from Vastus Lateralis muscle. Genes expression was measured by real-time PCR technique. There was a significant increase in PGC1α-1, PGC1α-4, IGF-1Ea and, IGF-1Eb mRNA after concentric contraction (p ≤ 0.05), while the PGC1α-4 and IGF-1Ec significantly increased after eccentric contraction (p ≤ 0.05). It is intriguing to highlight that; no significant differences between groups were evident for changes in any variables following exercise bouts (p ≥ 0.05). Our results found that concentric and eccentric contractions presented different responses in PGC1α-1, IGF-1Ea, IGF-1Eb, and IGF-1Ec mRNA. However, a similar significant increase in mRNA content was observed in PGC1α-4. Further, no apparent differences could be found between the response of genes to eccentric and concentric contraction. Keywords Eccentric contraction · Concentric contraction · Gene expression · PGC-1 alpha · IGF-1 · Myostatin Abbreviations CON Concentric ECC Eccentric GAPDH Glyceraldehyde 3-phosphate dehydrogenase HIIT High-intensity interval training IGF-1 Insulin-like growth factor-1 MGF Mechano growth factor mRNA Messenger ribonucleic acid MVIA Maximal voluntary isometric actions
* Mostafa Sabouri [email protected] 1
Department of Exercise Physiology & Health Science, University of Tehran, Tehran, Iran
2
Department of Pathology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
3
Shiraz Molecular Pathology Research Center, Dr. Daneshbod Lab, Shiraz, Iran
4
Shefa Neuroscience Research Center, Tehran, Iran
PGC-1 alpha Peroxisome proliferator-activated receptor gamma coactivator 1-alpha RE Resistance exercise RT-PCR Real-time polymerase chain reaction
Introduction Skeletal muscle synthesized and released myokines, which act as auto-, para- and endocrine mediators [1]. Myokines could affect non-muscle tissue and make crosstalk among muscles and other tissues. Moreover, skeletal muscle possesses high plasticity; therefore, it can undergo functional and morphological changes in response to different physical activity or disease conditions [2]. Acute and chronic muscle contraction eli
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