Effect of Hot-Pressing Temperature on the Subsequent Enzymatic Saccharification and Fermentation Performance of SPORL Pr
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Effect of Hot-Pressing Temperature on the Subsequent Enzymatic Saccharification and Fermentation Performance of SPORL Pretreated Forest Biomass Jingzhi Zhang & Andrea Laguna & Craig Clemons & Michael P. Wolcott & Rolland Gleisner & J. Y. Zhu & Xu Zhang
# Springer Science+Business Media New York (outside the USA) 2014
Abstract Methods to increase the energy density of biofuel feedstock for shipment are important towards improving supply chain efficiency in upstream processes. Towards this end, densified pretreated lignocellulosic biomass was produced using hot-pressing. The effects of fiber hornification induced by hot-pressing on enzymatic digestibilities of lodgepole pine and poplar NE222 wood chips pretreated by sulfite pretreatment to overcome recalcitrance of lignocelluloses (SPORL) were examined. Pretreated wood chips were pressed at 25, 70, 90, 110, and 177 °C. The cellulose accessibilities of the pressed and unpressed substrates were evaluated using water retention value and direct cellulase adsorption measurements. Hot-pressing below 110 °C produced a degree of hornification (DH) below 0.26 and had limited effect on cellulose accessibility and enzymatic digestibility. Hot-pressing at 177 °C produced a DH of 0.86 that substantially hornified the fibers and resulted near zero saccharification. The saccharification results were consistent with cellulose accessibility data. Ethanol fermentation studies at 18 % solids suggest that a
This work was conducted when J Zhang was a visiting student at the USDA Forest Products Lab and on official government time of Clemons, Gleisner, and Zhu. J. Zhang : X. Zhang School of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China J. Zhang : A. Laguna : C. Clemons : R. Gleisner : J. Y. Zhu (*) Forest Products Lab, USDA Forest Service, Madison, WI, USA e-mail: [email protected] A. Laguna University of Wisconsin-Madison, Madison, WI, USA M. P. Wolcott Department of Civil and Environmental Engineering, Washington State University, Pullman, WA, USA
pressing below 110 °C is preferred to reduce its effect on biofuel yield. Keywords Biomass commoditization . Supply chain logistics . Densification . Water retention value . Cellulase adsorption . Enzymatic hydrolysis
Introduction Economical biofuel production from lignocellulosic biomass will be facilitated by developing efficient methods to integrate the supply chain from feedstock to products [1, 2]. Feedstock logistics has a significant impact on many aspects of the process because it is a primary component of the fuel or chemical costs. In particular, this stage of the supply chain can dictate the facility scale and location, as well as the technology for conversion [3, 4]. The importance of biomass logistics is enhanced by the relatively low bulk density of original biomass. Even for woody biomass, the packing density of 75–200 kg/m3 and a moisture content of 50 % are common [3]. Transportation efficiency can be enhanced by densification and moisture reduction. One example of how these processes
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