Effect of Hydrogen Sulfide on Deformability of Rat Erythrocytes
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Bulletin of Experimental Biology and Medicine, Vol. 169, No. 6, October, 2020
725
PHYSIOLOGY Effect of Hydrogen Sulfide on Deformability of Rat Erythrocytes O. E. Fadyukova and V. B. Koshelev
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 169, No. 6, pp. 664-667, June, 2020 Original article submitted December 6, 2019 The influence of a hydrogen sulfide donor NaHS (2×10—5-10—3 M) on the rat erythrocyte deformability was analyzed by laser diffractometry. NaHS (6×10—5 M) increased, while NaHS (10—3 M) reduced erythrocyte deformability. The effect of NaHS (6×10—5 M) was similar to that of NO donor sodium nitroprusside (SNP, 10—7 M). However, simultaneous use of NaHS (6×10—5 M) and SNP induced less pronounced changes in erythrocyte deformability than their individual application. It is likely H2S, similar to NO, is involved in the regulation of erythrocyte deformability in the microvascular bed. Key Words: erythrocyte deformability; hydrogen sulfide; nitric oxide
The microrheological properties of red blood cells (aggregation and deformability) are the factors that determine blood viscosity and significantly affect blood flow in the microvascular bed. The deformability allows erythrocyte passage through capillaries of smaller diameter than their size, thus providing adequate blood supply to tissues [2,4]. The deformability of red blood cells in the bloodstream is influenced by a variety of bioactive substances, including gaseous transmitters: hydrogen sulfide H2S, carbon monoxide CO, and nitric oxide NO, released from the surrounding tissues and from red blood cells [2,3,11]. Effects of NO on the erythrocyte deformability are well studied [7,11], but the effects of H2S were not explored; the first data appeared recently [9]. Our aim was to study the effect of H2S donor sodium hydrogen sulfide NaHS on the deformability of rat erythrocytes and to compare it with the effect of NO donor sodium nitroprusside (SNP). Faculty of Fundamental Medicine, M. V. Lomonosov Moscow State University, Moscow, Russia. Address for correspondence: olefa@ hotmail.ru. O. E. Fadyukova
MATERIALS AND METHODS Fourteen white male rats weighing 375±30 g were used for the experiments. The animals were housed in standard cages and had unlimited access to water and food. The experiments were approved by the Bio ethics Committee of M. V. Lomonosov Moscow State University. The animals were anesthetized with chloral hydrate (450 mg/kg); sufficient depth of anesthesia was determined by the absence of the toe pinch reflex. The blood was sampled from the inferior vena cava. EDTA (2 mg/ml of blood, Sigma-Aldrich) was used as an anticoagulant. The blood from each rat was divided into aliquots and incubated with different concentrations of NaHS (2×10—5-10—3 M, Sigma-Aldrich) or with SNP (10—7 M, ICN Biomedicals) for 15 min at 37°С. The control blood sample was incubated with the same volume of the Ringer solution (solvent; in mM): 147 NaCl, 4 KCl, and 2.25 CaCl2 (pH 7.4). To prepare blood cell suspension, the blood samples were 50
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