In-silico identification and differential expression of putative disease resistance-related genes within the collinear r
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RESEARCH REPORT
In‑silico identification and differential expression of putative disease resistance‑related genes within the collinear region of Brassica napus blackleg resistance locus LepR2’ in Brassica oleracea Mohammad Rashed Hossain1,2 · Mostari Jahan Ferdous1 · Jong‑In Park1 · Arif Hasan Khan Robin1,2 · Sathishkumar Natarajan1 · Hee‑Jeong Jung1 · Hoy‑Taek Kim1 · Ill‑Sup Nou1 Received: 25 July 2019 / Revised: 16 May 2020 / Accepted: 7 July 2020 / Published online: 19 August 2020 © The Author(s) 2020
Abstract Blackleg disease, caused by Leptosphaeria maculans, greatly affects the production of cabbage (Brassica oleracea). However, definitive R-gene(s) are yet to be identified in this crop. In contrast, a number of R-loci have been identified in A- or B-genome crops. Identification of few resistant cabbage genotypes indicates the presence of R-genes in this C-genome crop. High ancestral synteny between Brassica genomes suggests that the collinear regions of known A- or B-genome R-loci may also contain functional R-genes in the C-genome. Strong resistance was observed in the cotyledons of cabbage inbred line SCNU-98 against two L. maculans isolates, 03–02 s and 00–100 s. We investigated the collinear region of the Brassica napus blackleg resistance locus LepR2’ in B. oleracea since both isolates of L. maculans contain corresponding avirulence genes. The locus was collinear to a 5.8 Mbp genomic segment of B. oleracea chromosome C09 containing 13 genes that have putative disease resistance-related domains. High expression of genes Bo9g117290 and Bo9g111510 against isolate 00–100 s, and high expression of genes Bo9g126150 and Bo9g111490 against both isolates in the resistant-line SCNU-98 indicate their putative roles in blackleg resistance, which remained to be functionally verified. This work enhances our understanding of R-gene-mediated resistance to blackleg in cabbage. Keywords Blackleg · Cabbage · LepR2’ · Leptosphaeria maculans · LRR-RLK · qRT-PCR · R-gene · Synteny
1 Introduction Blackleg, a disease particularly devastating to canola, is also known to cause substantial economic damage to cabbage (Dilmaghani et al. 2010; Humpherson-Jones 1985; Communicated by Inhwa Yeam. Mohammad Rashed Hossain and Mostari Jahan Ferdous contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13580-020-00271-5) contains supplementary material, which is available to authorized users. * Ill‑Sup Nou [email protected]; [email protected] 1
Department of Horticulture, Sunchon National University, Suncheon, Jeonnam 57922, Republic of Korea
Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensing 2202, Bangladesh
2
Piliponytė-Dzikienė et al. 2015). The disease is caused by Leptosphaeria maculans (anamorph: Phoma lingam) around the world and by Leptosphaeria biglobosa, a comparatively less damaging species, in Asian countries (Zhang et al. 2014; Liu et al. 2014; Hong et al. 2009; Hao et al. 2015; Mendes-Pereira et al.
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