Effect of methyl jasmonate on phenolic acids accumulation and the expression profile of their biosynthesis-related genes
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ORIGINAL ARTICLE
Effect of methyl jasmonate on phenolic acids accumulation and the expression profile of their biosynthesis‑related genes in Mentha spicata hairy root cultures Shirin Yousefian1 · Tahmineh Lohrasebi1 · Mohsen Farhadpour1 · Kamahldin Haghbeen1 Received: 31 March 2020 / Accepted: 26 May 2020 © Springer Nature B.V. 2020
Abstract Mentha spicata L., as a rich source of phenolic acids, is known for its therapeutic importance. Elicitors play a crucial role in biosynthetic pathways to improve plant secondary metabolites production. The purpose of this study was to investigate the effect of methyl jasmonate (MeJA) as a potent elicitor, on phenolic acids production and the relative changes in expression of their biosynthesis-related key genes (PAL, TAT, C4H, HPPR, and 4CL) in M. spicata hairy root cultures, to elucidate the interrelation between phenolic acids accumulation and the regulation mechanism of gene expression. The results showed that the relative expression levels of phenylpropanoid pathway genes, i.e., PAL, C4H, 4CL, and HPPR in the tyrosine-derived pathway increased 4.04-fold, 3.62-fold, 1.75-fold, and 1.45-fold in comparison to untreated controls, respectively. Highperformance liquid chromatography analysis indicated that MeJA dramatically increased rosmarinic acid content (55.44 µg g−1 dry wt, about 11.84-fold) after 6 h exposure to elicitor. Moreover, caffeic acid, chlorogenic acid, and cinnamic acid contents were also enhanced significantly (p
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