Effects of maternal dietary omega-3 polyunsaturated fatty acids and methionine during late gestation on fetal growth, DN
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RESEARCH
(2020) 11:111
Open Access
Effects of maternal dietary omega-3 polyunsaturated fatty acids and methionine during late gestation on fetal growth, DNA methylation, and mRNA relative expression of genes associated with the inflammatory response, lipid metabolism and DNA methylation in placenta and offspring’s liver in sheep Milca Rosa Velazquez1,2, Fernanda Batistel3, Juan Manuel Pinos Rodriguez1 and Alejandro Enrique Relling2*
Abstract Background: Omega-3 PUFA or methionine (Met) supply during gestation alters offspring physiology. However, the effect of both nutrients on fetal development has not been explored. Our objective was to determine the effects of supplementation of these two nutrients during late gestation on fetal growth, DNA methylation, and mRNA expression of genes associated with the inflammatory response, and DNA methylation. Ewes (n = 5/ treatment) were fed from day 100 to 145 of gestation one of the following treatments: 1) basal diet (NS) without fatty acids (FS) or methionine (MS) supplementation; 2) FS (10 g/kg Ca salts, source omega-3 PUFA); 3) MS (1 g/kg rumen protected methionine); and 4) FS and MS (FS-MS). On day 145, ewes were euthanized, and data from dams and fetus was recorded. Placenta (cotyledon), fetal liver, and blood samples were collected. (Continued on next page)
* Correspondence: [email protected] This material was partially presented in the 2019 American Society of Animal Science Association Annual Meeting 2 Department of Animal Science, Ohio Agricultural Research and Development Center (OARDC), The Ohio State University, 114 Gerlaugh Hall, 1680 Madison Ave, Wooster, OH 44691, USA Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Rosa Velazquez et al. Journal of Animal Science and Biotechnology
(2020) 11:111
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Results: A treatments interaction on fetal liver weight, ewe body weight and body condition score (BCS) was observed; FS-MS were
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