Electrochemical sensor based on dual-template molecularly imprinted polymer and nanoporous gold leaf modified electrode
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ORIGINAL PAPER
Electrochemical sensor based on dual-template molecularly imprinted polymer and nanoporous gold leaf modified electrode for simultaneous determination of dopamine and uric acid Nan Li 1 & Chuanchuan Nan 2 & Xuecui Mei 3 & Yudong Sun 3 & Huanhuan Feng 4,5 & Yingchun Li 3,6 Received: 16 February 2020 / Accepted: 23 June 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract A novel electrochemical sensor based on dual-template molecularly imprinted polymer (MIP) with nanoporous gold leaf (NPGL) was established for the simultaneous determination of dopamine (DA) and uric acid (UA). NPGL acts as an enlarged loading platform to enhance sensing capacity, and the MIP layer was synthesized in situ in the presence of monomer and dual templates (DA and UA) to provide specific recognition. Under the optimal conditions, the sensor shows a good linear range of 2.0~180 μM for DA at a working potential of 0.15 V (vs. Ag/AgCl) and 5.0~160 μM for UA at 0.35 V (vs. Ag/AgCl), with the respective detection limit of 0.3 μM and 0.4 μM (S/N = 3). Good selectivity of the sensor to its dual templates was confirmed as the sensing signals are significantly different between templates and interfering species. The responses maintained higher than 96% of the initial values after 30-day storage, and the day-to-day relative standard deviation is less than 3.0%. Real sample simultaneous determination of DA and UA was conducted with bovine serum, and the results were in good agreement with those from highperformance liquid chromatography. It can be concluded that this work offers a reliable, facile, fast, and cost-effective method of simultaneous quantification of two or more chem-/bio-molecules. Keywords Molecular imprinting technique . Nanomaterial . Biomarker . Electro-polymerization . Multiple targets
Introduction Dopamine (DA) is a neurotransmitter for message transfer and takes up an extremely important part in the renal and central
nervous system [1, 2]. Abnormal concentration of DA in vivo may cause several diseases, such as Parkinsonism [3] and Alzheimer’s disease [4]. Uric acid (UA) is the metabolic end product of purine nucleotide and the “garbage” of the human
Nan Li and Chuanchuan Nan contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04413-5) contains supplementary material, which is available to authorized users. * Huanhuan Feng [email protected] * Yingchun Li [email protected] 1
2
Department of Stomatology Center, Shenzhen People’s Hospital (Second Clinical Medical School of Jinan University; First Affiliated Hospital of Southern University of Science and Technology), Shenzhen 518020, Guangdong, China Department of ICU, Shenzhen People’s Hospital (Second Clinical Medical School of Jinan University; First Affiliated Hospital of Southern University of Science and Technology), Shenzhen 518020, Guangdong, China
3
College of Science, Harbin Institute of Technology (Shenzhen), Shenzhen 518055, Guang
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