Halovibrio salipaludis sp. nov., Isolated from Saline-Alkaline Soil
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Halovibrio salipaludis sp. nov., Isolated from Saline‑Alkaline Soil Liu Tang1,3 · Hanxu Liu1,3 · Wenni Sun2 · Juanjuan Zhao3 · Shaohua Miao3 · Qi Gong3 · Lian Ma1 · Guishan Zhang3 Received: 25 July 2020 / Accepted: 28 October 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract A Gram-staining-negative, halophilic, aerobic, oval-shaped or vibrio-shaped, motile by a polar flagellum strain, designated YL5-2T, was isolated from natural saline-alkaline wetland soil of Binhai new district, Tianjin, China. Strain YL5-2T grew optimally at 35 °C, pH 7.5–8.0, and in the presence of 10–25% (w/v) NaCl on MA medium. Phylogenetic analyses based on 16S rRNA gene sequences showed that the isolate belonged to the genus Halovibrio and exhibited high sequence similarity of 97.7% to Halovibrio variabilis DSM 3050T. The sole respiratory ubiquinone of strain YL5-2T is Q-9, and the dominant fatty acids were C18:1ω9c, C16:0, C19:0 cycloω8c, and Summed Feature 8. The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC), and lipid (L). The DNA G+C content of the strain was 62.1 mol%. The average nucleotide identity (ANI) based on whole genome sequences of strain YL5-2T and Halovibrio variabilis DSM 3 050T was 93.85%, and the dDDH value between these two strains was determined to be 52.0%. Phenotypic, chemotaxonomic, phylogenetic, and genomic analyses suggested that strain YL5-2T represent a novel species of the genus Halovibrio, for which the name Halovibrio salipaludis sp. nov. is proposed. The type strain is YL5-2T (=KCTC 52852T=ACCC 19971T).
Introduction The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene of strain YL5-2T is MF782425. The whole genome has been deposited at DDBJ/EMBL/GenBank under the accession NSKD00000000 for strain YL5-2T. Thin-layer chromatograms of the polar lipids extracted from strain YL5-2T and closely related species, additional phylogenetic trees, and the table containing the average nucleotide identity (ANI), and dDDH values to closely related genomes are available as Supplementary Materials. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02282-y) contains supplementary material, which is available to authorized users. * Lian Ma [email protected] * Guishan Zhang [email protected]; [email protected] 1
College of Life Science, Yangtze University, Hubei 434025, People’s Republic of China
2
China Bluestar Lehigh Engineering Institute Co., Ltd., Jiangsu 222004, People’s Republic of China
3
Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affair, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, People’s Republic of China
The genus Halovibrio was first proposed by Fendrich [1] and the type strain of Halovibrio variabilis was affiliated to strain DSM 3050T [2]. Up to now, only 2 speci
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